Molecular cloning and antimicrobial activity of bombolitin, a component of bumblebee Bombus ignitus venom

被引：36
作者：
Choo, Young Moo [1 ]
Lee, Kwang Sik [1 ]
Yoon, Hyung Joo [2 ]
Je, Yeon Ho [3 ]
Lee, Seon Woo [1 ]
Sohn, Hung Dae [1 ]
Jin, Byung Rae [1 ]
机构：
[1] Dong A Univ, Coll Nat Resources & Life Sci, Pusan 604714, South Korea
[2] RDA, Natl Acad Agncultural Sci, Dept Agr Biol, Suwon 441100, South Korea
[3] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2010年 / 156卷 / 03期
关键词：
Antimicrobial activity; Bombolitin; Bombus ignitus; Bumblebee; Venom; PHOSPHOLIPASE A(2); PORE FORMATION; PEPTIDES; MELITTIN; ANTIBACTERIAL; EXPRESSION; TRANSFERRIN; MEMBRANE;
D O I：
10.1016/j.cbpb.2010.03.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bombolitin is the most abundant component of bumblebee venom and shares structural and biological properties with melittin, a component of honeybee venom Here, we describe the molecular cloning and antimicrobial activity of bombolitin isolated from the venom of the bumblebee Bombus ignitus The B. ignitus bombolitin gene consists of 2 exons encoding 56-amino acid residues The bombolitin isolated from B ignitus venom is a 1.99 kDa mature peptide with 18-amino acid residues, and It is created by the cleavage of the probombolitin domain between Ala38 and Leu39 B ignitus bombolitin exhibits venom gland-specific expression We also investigated the antimicrobial properties of B ignitus bombolitin against bacteria and fungi. The B ignitus bombolitin showed high antibacterial activity against two Gram-positive and two Gram-negative bacteria. In addition, the B ignitus bombolitin displayed antifungal activity against the plant pathogenic fungi Fulvia fulva and Alternaria radicina. (C) 2010 Elsevier Inc All rights reserved.
引用
收藏
页码：168 / 173
页数：6
相关论文
共 30 条
  • [21] Membrane active antimicrobial activity and molecular dynamics study of a novel cationic antimicrobial peptide polybia-MPI, from the venom of Polybia paulista
    Wang, Kairong
    Yan, Jiexi
    Dang, Wen
    Liu, Xin
    Chen, Ru
    Zhang, Jindao
    Zhang, Bangzhi
    Zhang, Wei
    Kai, Ming
    Yan, Wenjin
    Yang, Zhibin
    Xie, Junqiu
    Wang, Rui
    PEPTIDES, 2013, 39 : 80 - 88
  • [22] Molecular cloning and antimicrobial activity of enterolysin A and helveticin J of bacteriolysins from metagenome of Chinese traditional fermented foods
    Zhang, Tiantian
    Pan, Yingjie
    Li, Bailin
    Ou, Jie
    Zhang, Jianmin
    Chen, Yourong
    Peng, Xu
    Chen, Lanming
    FOOD CONTROL, 2013, 31 (02) : 499 - 507
  • [23] Characterization of the venom from the Australian scorpion Urodacus yaschenkoi: Molecular mass analysis of components, cDNA sequences and peptides with antimicrobial activity
    Luna-Ramirez, Karen
    Quintero-Hernandez, Veronica
    Vargas-Jaimes, Leone
    Batista, Cesar V. F.
    Winkel, Kenneth D.
    Possani, Lourival D.
    TOXICON, 2013, 63 : 44 - 54
  • [24] Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus
    Liu, Yuan
    Cui, Zhaoxia
    Li, Xihong
    Song, Chengwen
    Li, Qianqian
    Wang, Shuangyan
    FISH & SHELLFISH IMMUNOLOGY, 2012, 33 (01) : 85 - 91
  • [25] Purification, molecular cloning, and antimicrobial activity of peptides from the skin secretion of the black-spotted frog, Rana nigromaculata
    Li, Ang
    Zhang, Yong
    Wang, Che
    Wu, Geng
    Wang, Zhenchun
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2013, 29 (10) : 1941 - 1949
  • [26] Molecular cloning, genomic structure and antimicrobial activity of PtALF7, a unique isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus
    Liu, Yuan
    Cui, Zhaoxia
    Li, Xihong
    Song, Chengwen
    Shi, Guohui
    Wang, Chunlin
    FISH & SHELLFISH IMMUNOLOGY, 2013, 34 (02) : 652 - 659
  • [27] Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds
    Slavokhotova, Anna A.
    Odintsova, Tatyana I.
    Rogozhin, Eugene A.
    Musolyamov, Alexander K.
    Andreev, Yaroslav A.
    Grishin, Eugene V.
    Egorov, Tsezi A.
    BIOCHIMIE, 2011, 93 (03) : 450 - 456
  • [28] Molecular cloning, expression of a big defensin gene from bay scallop Argopecten irradians and the antimicrobial activity of its recombinant protein
    Zhao, Jianmin
    Song, Linsheng
    Li, Chenghua
    Ni, Duojiao
    Wu, Longtao
    Zhu, Ling
    Wang, Hao
    Xu, Wei
    MOLECULAR IMMUNOLOGY, 2007, 44 (04) : 360 - 368
  • [29] An Expedient Three-component Synthesis of Novel Pyrido-pyrimidine Derivatives: Antimicrobial Activity, Molecular Docking, and ADME Studies
    Rai, Sonam
    Bishnoi, Abha
    Fatma, Shaheen
    Shukla, Shraddha
    Devi, Poornima
    Singh, Vineeta
    POLYCYCLIC AROMATIC COMPOUNDS, 2024, 44 (04) : 2752 - 2774
  • [30] Molecular Cloning, Recombinant Expression, and Antimicrobial Activity of EC-hepcidin3, a New Four-Cysteine Hepcidin Isoform from Epinephelus coioides
    Qu, HaiDong
    Chen, Bei
    Peng, Hui
    Wang, KeJian
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2013, 77 (01) : 103 - 110
123