Intracellular visualization of tetrodotoxin (TTX) in the skin of a puffer Tetraodon nigroviridis by immunoenzymatic technique

被引：38

作者：

Mahmud, Y

Arakawa, O ^{[1
]}

Ichinose, A

Tanu, MB

Takatani, T

Tsuruda, K

Kawatsu, K

Hamano, Y

Noguchi, T

机构：

[1] Nagasaki Univ, Fac Fisheries, Nagasaki 8528521, Japan

[2] Nagasaki Univ, Inst Trop Med, Electron Microscope Cent Lab, Nagasaki 8528523, Japan

[3] Nagasaki Univ, Grad Sch Sci & Technol, Nagasaki 8528521, Japan

[4] Japan Frozen Foods Inspect Corp, Tokyo 1050012, Japan

[5] Osaka Prefectural Inst Publ Hlth, Dept Food Microbiol, Osaka 5370025, Japan

来源：

TOXICON | 2003年 / 41卷 / 05期

基金：

日本学术振兴会;

关键词：

micro-distribution; tetrodotoxin; electron microscopy; basal cell; lysosome; monoclonal antibody; immunogold;

D O I：

10.1016/S0041-0101(03)00003-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Micro-distributions of tetrodotoxin (TTX) in the skin of a brackishwater puffer Tetraodon nigroviridis were investigated by means of a monoclonal anti-TTX antibody under light and transmission electron microscope. In light microscopy TTX antigen was visualized as brown color in undifferentiated basal cells and succiform cells of the skin, while in electron microscopy TTX was detected as black dots of immunogold in lysosomes of basal cells. From the results, it can be inferred that when TTX from the blood plasma of T. nigroviridis enters into the undifferentiated basal cells through diffusion, it is taken to the lysosomes by phagocytosis and exists there through binding with internal constituent(s) of the organelles. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：605 / 611

页数：7

共 25 条

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