Identification and Functional Analysis of the CLAVATA3/EMBRYO SURROUNDING REGION (CLE) Gene Family in Wheat

被引:27
作者
Li, Zheng [1 ]
Liu, Dan [1 ]
Xia, Yu [1 ]
Li, Ziliang [1 ]
Niu, Na [1 ]
Ma, Shoucai [1 ]
Wang, Junwei [1 ]
Song, Yulong [1 ]
Zhang, Gaisheng [1 ]
机构
[1] Northwest A&F Univ, Natl Yangling Agr Biotechnol & Breeding Ctr, Wheat Breeding Engn Res Ctr,State Wheat Improveme, Coll Agron,Yangling Branch,Minist Educ,Key Lab Cr, Yangling 712100, Shaanxi, Peoples R China
关键词
CLE peptide; function analysis; pre-propeptides; mature peptides; wheat; OF-FUNCTION PHENOTYPES; ROOT-MERISTEM GROWTH; RECEPTOR-LIKE KINASE; ENDOSPERM DEVELOPMENT; SIGNALING MOLECULES; ARABIDOPSIS; PEPTIDES; SHOOT; CLV3; POLYPEPTIDE;
D O I
10.3390/ijms20174319
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are post-translationally cleaved and modified peptides from their corresponding pre-propeptides. Although they are only 12 to 13 amino acids in length, they are important ligands involved in regulating cell proliferation and differentiation in plant shoots, roots, vasculature, and other tissues. They function by interacting with their corresponding receptors. CLE peptides have been studied in many plants, but not in wheat. We identified 104 TaCLE genes in the wheat genome based on a genome-wide scan approach. Most of these genes have homologous copies distributed on sub-genomes A, B, and D. A few genes are derived from tandem duplication and segmental duplication events. Phylogenetic analysis revealed that TaCLE genes can be divided into five different groups. We obtained functional characterization of the peptides based on the evolutionary relationships among the CLE peptide families of wheat, rice, and Arabidopsis, and expression pattern analysis. Using chemically synthesized peptides (TaCLE3p and TaCLE34p), we found that TaCLE3 and TaCLE34 play important roles in regulating wheat and Arabidopsis root development, and wheat stem development. Overexpression analysis of TaCLE3 in Arabidopsis revealed that TaCLE3 not only affects the development of roots and stems, but also affects the development of leaves and fruits. These data represent the first comprehensive information on TaCLE family members.
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页数:16
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