New aspects of the simultaneous analysis of amino acids and amines as their o-phthaldialdehyde derivatives by high-performance liquid chromatography -: Analysis of wine, beer and vinegar

被引：107

作者：

Kutlán, D ^{[1
]}

Molnár-Perl, I ^{[1
]}

机构：

[1] Eotvos Lorand Univ, Inst Inorgan & Analyt Chem, H-1443 Budapest 112, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 987卷 / 1-2期

关键词：

wine; beer; food analysis derivatization; LC; amino acids; amines;

D O I：

10.1016/S0021-9673(02)01538-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new high-performance liquid chromatography method is described for the simultaneous quantitation of amino acids and amines for 37 compounds (20 amino acids+17 amines), as their o-phthaldialdehyde (OPA)-3-mercaptopropionic acid derivatives, within 53 min. Based on previously documented stoichiometric and reaction mechanism studies, derivatizations have been carried out with the OPA-SH-group= 1:50 containing reagents. Reliability and reproducibility of analyses have been considerably improved. Average reproducibility data in a wide concentration range of derivatives had RSDless than or equal to3.4%. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：311 / 322

页数：12

共 9 条

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JOURNAL OF CHROMATOGRAPHY A, 2001, 913 (1-2) :283-302

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