The Eukaryotic Elongation Factor 1A Is Critical for Genome Replication of the Paramyxovirus Respiratory Syncytial Virus

被引：21

作者：

Wei, Ting ^{[1
]}

Li, Dongsheng ^{[1
]}

Marcial, Daneth ^{[1
,2
,3
]}

Khan, Moshin ^{[1
,3
]}

Lin, Min-Hsuan ^{[1
]}

Snape, Natale ^{[2
,3
]}

Ghildyal, Reena ^{[4
]}

Harrich, David ^{[1
,5
]}

Spann, Kirsten ^{[2
,3
,5
]}

机构：

[1] Queensland Inst Med Res Berghofer, Herston, Qld, Australia

[2] Univ Queensland, Clin Med Virol Ctr, Herston, Qld, Australia

[3] Childrens Hlth Queensland, Sir Albert Sakzewski Virus Res Ctr, Herston, Qld, Australia

[4] Univ Canberra, Ctr Res Therapeut Solut, Canberra, ACT 2601, Australia

[5] Australian Infect Dis Res Ctr, St Lucia, Qld, Australia

来源：

PLOS ONE | 2014年 / 9卷 / 12期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

DEPENDENT RNA-POLYMERASE; FACTOR-I; MATRIX PROTEIN; INFECTED-CELLS; VIRAL-RNA; EF-TU; TRANSLATION; ALPHA; INTERACTS; COMPLEX;

D O I：

10.1371/journal.pone.0114447

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The eukaryotic translation factor eEF1A assists replication of many RNA viruses by various mechanisms. Here we show that down-regulation of eEF1A restricts the expression of viral genomic RNA and the release of infectious virus, demonstrating a biological requirement for eEF1A in the respiratory syncytial virus (RSV) life cycle. The key proteins in the replicase/transcriptase complex of RSV; the nucleocapsid (N) protein, phosphoprotein (P) and matrix (M) protein, all associate with eEF1A in RSV infected cells, although N is the strongest binding partner. Using individually expressed proteins, N, but not P or M bound to eEF1A. This study demonstrates a novel interaction between eEF1A and the RSV replication complex, through binding to N protein, to facilitate genomic RNA synthesis and virus production.

引用

页数：14

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