Detection of immune complexes using atomic force Microscopy

被引：0
作者：
Malyuchenko, NV
Agapov, II
Tonevitsky, AG
Moisenovich, MM
Savvateev, MN
Tonevitsky, EA
Bykov, VA
Kiirpichnikov, MP
机构：
[1] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119992, Russia
[2] NTMDT Co, State Res Inst Phys Problems, Moscow 124482, Russia
来源：
BIOFIZIKA | 2004年 / 49卷 / 06期
关键词：
atomic force microscopy; semicontact mode; antibodies; viscumin; ricin;
D O I：
暂无
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Complex formation between immunoglobulins and ligands immobilized on mica was studied by atomic force microscopy in two different systems. In the first system, 60-kDa ligands possessing only one site for antibody recognition were used. In the other system, a more complex interaction of human immunoglobulin with immobilized polyclonal antibodies was studied. In both systems, specific complexes with proper ligand appeared, and unspecific interaction was not detected. The method of revealing immunocomplexes by image atomic force microscopy can be used in the development of modern diagnostic systems.
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页码：1008 / 1014
页数：7
相关论文
共 12 条
[1]   Biomolecular force measurements and the atomic force microscope [J].
Allison, DP ;
Hinterdorfer, P ;
Han, WH .
CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) :47-51
[2]   RIBOSOME-INACTIVATING PROTEINS FROM PLANTS [J].
BARBIERI, L ;
BATTELLI, MG ;
STIRPE, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1154 (3-4) :237-282
[3]   Atomic force microscopy as a tool for biomedical and biotechnological studies [J].
Cidade, GAG ;
Costa, LT ;
Weissmüller, G ;
Neto, AJD ;
Roberty, NC ;
de Moraes, MB ;
Prazeres, GMP ;
Hill, CEM ;
Ribeiro, SJM ;
de Souza, GGB ;
Teixeira, LDP ;
Monçores, MD ;
Bisch, PM .
ARTIFICIAL ORGANS, 2003, 27 (05) :447-451
[4]   Atomic-force microscopy: Rhodopsin dimers in native disc membranes [J].
Fotiadis, D ;
Liang, Y ;
Filipek, S ;
Saperstein, DA ;
Engel, A ;
Palczewski, K .
NATURE, 2003, 421 (6919) :127-128
[5]   From cell imaging to molecular manipulation: biological applications of atomic force microscopy [J].
Giocondi, MC ;
Milhiet, PE ;
Lesniewska, E ;
Le Grimellec, C .
M S-MEDECINE SCIENCES, 2003, 19 (01) :92-99
[6]   ATOMIC FORCE MICROSCOPY IMAGING OF DOUBLE-STRANDED DNA AND RNA [J].
LYUBCHENKO, YL ;
GALL, AA ;
SHLYAKHTENKO, LS ;
HARRINGTON, RE ;
JACOBS, BL ;
ODEN, PI ;
LINDSAY, SM .
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1992, 10 (03) :589-606
[7]  
Malyuchenko NV, 2003, BIOFIZIKA+, V48, P830
[8]   High-resolution imaging of antibodies by tapping-mode atomic force microscopy:: Attractive and repulsive tip-sample interaction regimes [J].
San Paulo, A ;
García, R .
BIOPHYSICAL JOURNAL, 2000, 78 (03) :1599-1605
[9]  
SAVVATEEV MN, 2003, AIP C P, V696, P137
[10]  
Tonevitsky AG, 1999, ARZNEIMITTELFORSCH, V49, P970
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