Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells

被引:12
作者
Martinez-Alonso, Carmen [1 ]
Taroncher, Mercedes [1 ]
Castaldo, Luigi [2 ]
Izzo, Luana [2 ]
Rodriguez-Carrasco, Yelko [1 ]
Ritieni, Alberto [2 ]
Ruiz, Maria-Jose [1 ]
机构
[1] Univ Valencia, Fac Pharm, Dept Prevent Med & Publ Hlth Food Sci Toxicol & F, Valencia 46100, Spain
[2] Univ Naples Federico II, Fac Pharm, Dept Pharm, Via Domenico Montesano 49, I-80131 Naples, Italy
关键词
beans; phenolic compounds; T-2; toxin; HepG2; cells; reactive oxygen species; ANTIOXIDANT ACTIVITY; BIOACTIVE COMPOUNDS; POLYPHENOLS;
D O I
10.3390/foods11071033
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was performed using a Q-Orbitrap high-resolution mass spectrometry analysis. Notably, epicatechin and delphinidin were the most detected polyphenols found in red bean extracts (3.297 and 3.108 mg/Kg, respectively). Moreover, the red bean extract was evaluated against the T-2 toxin (T-2) induced cytotoxicity in hepatocarcinoma cells (HepG2) by direct treatment, simultaneous treatment, and pre-treatment assays. These data showed that T-2 affected the cell viability in a dose-dependent manner, as well as observing a cytotoxic effect and a significant increase in ROS production at 30 nM. The simultaneous treatment and the pre-treatment of HepG2 cells with red bean extract was not able to modify the cytotoxic T-2 effect. However, the simultaneous treatment of T-2 at 7.5 nM with the red bean extract showed a significant decrease in ROS production, with respect to the control. These results suggest that the red bean extract could modulate oxidative stress on HepG2 cells.
引用
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页数:15
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