Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells

被引:10
|
作者
Martinez-Alonso, Carmen [1 ]
Taroncher, Mercedes [1 ]
Castaldo, Luigi [2 ]
Izzo, Luana [2 ]
Rodriguez-Carrasco, Yelko [1 ]
Ritieni, Alberto [2 ]
Ruiz, Maria-Jose [1 ]
机构
[1] Univ Valencia, Fac Pharm, Dept Prevent Med & Publ Hlth Food Sci Toxicol & F, Valencia 46100, Spain
[2] Univ Naples Federico II, Fac Pharm, Dept Pharm, Via Domenico Montesano 49, I-80131 Naples, Italy
关键词
beans; phenolic compounds; T-2; toxin; HepG2; cells; reactive oxygen species; ANTIOXIDANT ACTIVITY; BIOACTIVE COMPOUNDS; POLYPHENOLS;
D O I
10.3390/foods11071033
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was performed using a Q-Orbitrap high-resolution mass spectrometry analysis. Notably, epicatechin and delphinidin were the most detected polyphenols found in red bean extracts (3.297 and 3.108 mg/Kg, respectively). Moreover, the red bean extract was evaluated against the T-2 toxin (T-2) induced cytotoxicity in hepatocarcinoma cells (HepG2) by direct treatment, simultaneous treatment, and pre-treatment assays. These data showed that T-2 affected the cell viability in a dose-dependent manner, as well as observing a cytotoxic effect and a significant increase in ROS production at 30 nM. The simultaneous treatment and the pre-treatment of HepG2 cells with red bean extract was not able to modify the cytotoxic T-2 effect. However, the simultaneous treatment of T-2 at 7.5 nM with the red bean extract showed a significant decrease in ROS production, with respect to the control. These results suggest that the red bean extract could modulate oxidative stress on HepG2 cells.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] T-2 toxin-induced cytotoxicity and damage on TM3 Leydig cells
    Yuan, Zhihang
    Matias, Froilan Bernard
    Yi, Jin-e
    Wu, Jing
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2016, 181 : 47 - 54
  • [2] Enzymatic defense of HepG2 cells against cytotoxicity induced by T-2 and its modified forms
    Taroncher, M.
    Rodriguez-Carrasco, Y.
    Ruiz, M. J.
    TOXICOLOGY LETTERS, 2021, 350 : S106 - S106
  • [3] TET3-mediated DNA demethylation and chromatin remodeling regulate T-2 toxin-induced human CYP1A1 expression and cytotoxicity in HepG2 cells
    Zhu, Jiahui
    Li, Guihong
    Huang, Qiang
    Wen, Jikai
    Deng, Yiqun
    Jiang, Jun
    BIOCHEMICAL PHARMACOLOGY, 2023, 211
  • [4] Cytotoxicity and Genotoxicity of Senecio vulgaris L. Extracts: An In Vitro Assessment in HepG2 Liver Cells
    Acito, Mattia
    Russo, Carla
    Fatigoni, Cristina
    Mercanti, Federica
    Moretti, Massimo
    Villarini, Milena
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2022, 19 (22)
  • [5] Protective Effect of Selenomethionine on T-2 Toxin-Induced Rabbit Immunotoxicity
    Zhang, Ziqiang
    Xu, Yaolu
    Wang, Jiajia
    Xie, Hui
    Sun, Xueyan
    Zhu, Xuemin
    Wei, Lan
    Liu, Yumei
    BIOLOGICAL TRACE ELEMENT RESEARCH, 2022, 200 (01) : 172 - 182
  • [6] Interactions between T-2 toxin and its metabolites in HepG2 cells and in silico approach
    Taroncher, Mercedes
    Rodriguez-Carrasco, Yelko
    Ruiz, Maria-Jose
    FOOD AND CHEMICAL TOXICOLOGY, 2021, 148
  • [7] T-2 toxin-induced apoptosis in intestinal crypt epithelial cells of mice
    Li, GM
    Shinozuka, J
    Uetsuka, K
    Nakayama, H
    Doi, K
    EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, 1997, 49 (06) : 447 - 450
  • [8] Cytotoxicity and apoptosis induced by T-2 toxin in cultured Vero cells
    Bouaziz, C.
    Abid, S.
    Bouslimi, A.
    El Golli, E.
    Bacha, H.
    FEBS JOURNAL, 2006, 273 : 73 - 73
  • [9] Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
    Taroncher, Mercedes
    Fuentes, Cristina
    Rodriguez-Carrasco, Yelko
    Ruiz, Maria-Jose
    TOXICOLOGY, 2024, 501
  • [10] Antidiabetic components from the hexane extract of red kidney beans (Phaseolus vulgaris L.): isolation and structure determination
    Sutedja, Anita Maya
    Yanase, Emiko
    Batubara, Irmanida
    Fardiaz, Dedi
    Lioe, Hanifah Nuryani
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2020, 84 (03) : 598 - 605