Gellan Gum-Hyaluronic Acid Spongy-like Hydrogels and Cells from Adipose Tissue Synergize Promoting Neoskin Vascularization

被引:83
作者
Cerqueira, Mariana Teixeira [1 ,2 ]
da Silva, Lucilia Pereira [1 ,2 ]
Santos, Tircia Carlos [1 ,2 ]
Pirraco, Rogerio Pedro [1 ,2 ]
Correlo, Vitor Manuel [1 ,2 ]
Reis, Rui Luis [1 ,2 ]
Marques, Alexandra Pinto [1 ,2 ]
机构
[1] Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Headquarters European Inst Excellence Tissue Engn, P-4806909 Taipas, Guimaraes, Portugal
[2] PT Govt Associate Lab, ICVS 3Bs, Braga, Portugal
关键词
skin; tissue engineering; gellan gum; hyaluronic acid; vascularization; stem cells; MESENCHYMAL STEM-CELLS; IN-VITRO RECONSTRUCTION; EXTRACELLULAR-MATRIX; ENDOTHELIAL-CELLS; SKIN SUBSTITUTE; SCAFFOLDS; ANGIOGENESIS; REGENERATION; NETWORK; GRAFTS;
D O I
10.1021/am504520j
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Currently available substitutes for skin wound healing often result in the formation of nonfunctional neotissue. Thus, urgent care is still needed to promote an effective and complete regeneration. To meet this need, we proposed the assembling of a construct that takes advantage of cell-adhesive gellan gum-hyaluronic acid (GG-HA) spongy-like hydrogels and a powerful cell-machinery obtained from adipose tissue, human adipose stem cells (hASCs), and microvascular endothelial cells (hAMECs). In addition to a cell-adhesive character, GG-HA spongy-like hydrogels overpass limitations of traditional hydrogels, such as reduced physical stability and limited manipulation, due to improved microstructural arrangement characterized by pore wall thickening and increased mean pore size. The proposed constructs combining cellular mediators of the healing process within the spongy-like hydrogels that intend to recapitulate skin matrix aim to promote neoskin vascularization. Stable and off-the-shelf dried GG-HA polymeric networks, rapidly rehydrated at the time of cell seeding then depicting features of both sponges and hydrogels, enabled the natural cell entrapment/encapsulation and attachment supported by cell-polymer interactions. Upon transplantation into mice full-thickness excisional wounds, GG-HA spongy-like hydrogels absorbed the early inflammatory cell infiltrate and led to the formation of a dense granulation tissue. Consequently, spongy-like hydrogel degradation was observed, and progressive wound closure, re-epithelialization, and matrix remodelling was improved in relation to the control condition. More importantly, GG-HA spongy-like hydrogels promoted a superior neovascularization, which was enhanced in the presence of human hAMECs, also found in the formed neovessels. These observations highlight the successful integration of a valuable matrix and prevascularization cues to target angiogenesis/neovascularization in skin full-thickness excisional wounds.
引用
收藏
页码:19668 / 19679
页数:12
相关论文
共 67 条
  • [61] The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice
    Wang, Tzu-Wei
    Sun, Jui-Sheng
    Wu, Hsi-Chin
    Tsuang, Yang-Hwei
    Wang, Wen-Hsi
    Lin, Feng-Huei
    [J]. BIOMATERIALS, 2006, 27 (33) : 5689 - 5697
  • [62] Effects of steroids and retinoids on wound healing
    Wicke, C
    Halliday, B
    Allen, D
    Roche, NS
    Scheuenstuhl, H
    Spencer, MM
    Roberts, AB
    Hunt, TK
    [J]. ARCHIVES OF SURGERY, 2000, 135 (11) : 1265 - 1270
  • [63] Evaluation of ultra-thin poly(ε-caprolactone) films for tissue-engineered skin
    Woei, K
    Hutmacher, DW
    Schantz, JT
    Seng, C
    Too, HP
    Chye, T
    Phan, TT
    Teoh, SH
    [J]. TISSUE ENGINEERING, 2001, 7 (04): : 441 - 455
  • [64] Wong VW, 2011, TISSUE ENG PT A, V17, P631, DOI [10.1089/ten.tea.2010.0298, 10.1089/ten.TEA.2010.0298]
  • [65] Tissue-engineered microvessels on three-dimensional biodegradable scaffolds using human endothelial progenitor cells
    Wu, X
    Rabkin-Aikawa, E
    Guleserian, KJ
    Perry, TE
    Masuda, Y
    Sutherland, FWH
    Schoen, FJ
    Mayer, JE
    Bischoff, J
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2004, 287 (02): : H480 - H487
  • [66] Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis
    Wu, Yaojiong
    Chen, Liwen
    Scott, Paul G.
    Tredget, Edward E.
    [J]. STEM CELLS, 2007, 25 (10) : 2648 - 2659
  • [67] SYNTHESIS AND CHARACTERIZATION OF A MODEL EXTRACELLULAR-MATRIX THAT INDUCES PARTIAL REGENERATION OF ADULT MAMMALIAN SKIN
    YANNAS, IV
    LEE, E
    ORGILL, DP
    SKRABUT, EM
    MURPHY, GF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (03) : 933 - 937