The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer

被引：55

作者：

Satake, Hirofumi ^{[1
]}

Tamura, Kenji ^{[1
]}

Furihata, Mutsuo ^{[2
]}

Anchi, Takashi ^{[1
]}

Sakoda, Hatsune ^{[1
]}

Kawada, Chiaki ^{[1
]}

Iiyama, Tatsuo ^{[3
]}

Ashida, Shingo ^{[1
]}

Shuin, Taro ^{[1
]}

机构：

[1] Kochi Univ, Dept Urol, Kochi Med Sch, Nanko Ku, Kochi 7838505, Japan

[2] Kochi Univ, Dept Pathol, Kochi Med Sch, Nanko Ku, Kochi 7838505, Japan

[3] Kochi Univ, Dept Immunol, Kochi Med Sch, Nanko Ku, Kochi 7838505, Japan

来源：

ONCOLOGY REPORTS | 2010年 / 23卷 / 01期

关键词：

interferon-stimulated gene 15; cDNA microarray; prostate cancer; TUMOR-CELLS; PROTEIN; ISG15; MITOXANTRONE; PREDNISONE; EXPRESSION; DOCETAXEL;

D O I：

10.3892/or_00000600

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To identify molecules to serve as diagnostic markers for high-grade prostate cancer (PC) and targets for novel therapeutic drugs, we investigated the gene expression profiles of high-grade PCs using a cDNA microarray combined with laser microbeam microdissection. We subsequently confirmed that the ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) was expressed exclusively in high-grade PCs with high Gleason scores. Semi-quantitative reverse transcription PCR and immunohistochemical analysis confirmed the overexpression of ISG15, a 165 amino acid interferon-inducible ubiquitin-like protein, specifically in high-grade PCs with high Gleason scores 8-9, while it was not expressed in the normal prostate. Immunohistochemical analysis using anti-ISG15 polyclonal antibody confirmed an elevated expression of ISG15 protein in high-grade PCs as well as low-grade PCs compared with that in normal prostate (NP) epithelium. Knockdown of ISG15 expression by short interfering RNA (siRNA) in a PC cell line resulted in marked attenuation of PC cell survival; concordantly, ISG15 overexpression in a PC cell line promoted PC cell growth, indicating its oncogenic property. These findings suggest that ISG15 is involved in cell growth and survival of PCs and that it could be a potential molecular target for new therapeutics and a diagnostic biomarker for human PCs.

引用

页码：11 / 16

页数：6

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