CREB, a possible upstream regulator of Bcl-2 in trichosanthin-induced HeLa cell apoptosis

被引：22

作者：

Wang, Ping ^{[1
]}

Xu, Jin ^{[1
]}

Zhang, Cheng ^{[1
]}

机构：

[1] Ningbo Univ, Sch Med, Ningbo 315211, Zhejiang, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 04期

基金：

中国国家自然科学基金;

关键词：

CREB; Bcl-2; expression; Apoptosis; HeLa cell; Trichosanthin; ELEMENT-BINDING PROTEIN; TRANSCRIPTION FACTOR; CHORIOCARCINOMA CELLS; EXPRESSION; PROMOTER; CAMP; NEURONS; FAMILY; GROWTH;

D O I：

10.1007/s11033-009-9629-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our previous reports indicated that cyclic AMP response element-binding (CREB) protein was involved in the regulation of Bcl-2 expression in apoptotic HeLa cells induced by trichosanthin (TCS). Here we presented that blockade the binding site of CREB to Bcl-2 by a CRE decoy oligonucleotide abrogated the TCS-decreased Bcl-2 expression. Furthermore, overexpression of phosphorylated CREB (p-CREB) in cells transfected with p-CREB/GFP fusion construct resulted in an increase of Bcl-2 protein content, however, this increase was attenuated by TCS treatment. Therefore, this data supports the hypothesis that CREB is a possible upstream regulator of Bcl-2 in apoptotic HeLa cells induced by TCS. The study provides new insights into understanding the mechanism of TCS in the treatment of cervical cancer.

引用

页码：1891 / 1896

页数：6

共 22 条

[1] BCL-2 FAMILY: Regulators of cell death [J].

Chao, DT ;

Korsmeyer, SJ .

ANNUAL REVIEW OF IMMUNOLOGY, 1998, 16 :395-419

[2] Characterization of the mouse adenylyl cyclase type VIII gene promoter:: regulation by cAMP and CREB [J].

Chao, JR ;

Ni, YG ;

Bolaños, CA ;

Rahman, Z ;

DiLeone, RJ ;

Nestler, EJ .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2002, 16 (07) :1284-1294

[3] The cyclic AMP response element in the Bcl-2 promoter confers inducibility by hypoxia in neuronal cells [J].

Freeland, K ;

Boxer, LM ;

Latchman, DS .

MOLECULAR BRAIN RESEARCH, 2001, 92 (1-2) :98-106

[4] CYCLIC-AMP STIMULATES SOMATOSTATIN GENE-TRANSCRIPTION BY PHOSPHORYLATION OF CREB AT SERINE-133 [J].

GONZALEZ, GA ;

MONTMINY, MR .

CELL, 1989, 59 (04) :675-680

[5] CREB is required for acquisition of ischemic tolerance in gerbil hippocampal CA1 region [J].

Hara, T ;

Hamada, J ;

Yano, S ;

Morioka, M ;

Kai, Y ;

Ushio, Y .

JOURNAL OF NEUROCHEMISTRY, 2003, 86 (04) :805-814

[6] CREB proteins function as positive regulators of the translocated bcl-2 allele in t(14;18) lymphomas [J].

Ji, L ;

Mochon, E ;

Arcinas, M ;

Boxer, LM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (37) :22687-22691

[7]

MARAGANORE JM, 1987, J BIOL CHEM, V262, P11628

[8] CREB-mediated Bcl-2 protein expression after ischemic preconditioning [J].

Meller, R ;

Minami, M ;

Cameron, JA ;

Impey, S ;

Chen, DX ;

Lan, JQ ;

Henshall, DC ;

Simon, RP .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2005, 25 (02) :234-246

[9] Regulation of p73 by Hck through kinase-dependent and independent mechanisms [J].

Paliwal, Preeti ;

Radha, Vegesna ;

Swarup, Ghanshyam .

BMC MOLECULAR BIOLOGY, 2007, 8

[10] Dual blockade of cyclic AMP response element- (CRE) and AP-1-directed transcription by CRE-transcription factor decoy oligonucleotide - Gene-specific inhibition of tumor growth [J].

Park, YG ;

Nesterova, M ;

Agrawal, S ;

Cho-Chung, YS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (03) :1573-1580

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