Tissue distribution, bioconcentration, metabolism, and effects of erythromycin in crucian carp (Carassius auratus)

被引：89
作者：
Liu, Jianchao [1 ]
Lu, Guanghua [1 ]
Ding, Jiannan [1 ]
Zhang, Zhenghua [1 ]
Wang, Yonghua [1 ]
机构：
[1] Hohai Univ, Coll Environm, Key Lab Integrated Regulat & Resources Dev Shallo, Nanjing 210098, Jiangsu, Peoples R China
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2014年 / 490卷
基金：
中国国家自然科学基金;
关键词：
Erythromycin; Metabolites; Bioconcentration; Biomarker; ACETYLCHOLINESTERASE ACTIVITY; OXIDATIVE STRESS; BIOACCUMULATION; PHARMACEUTICALS; ANTIBIOTICS; EXPOSURE; CARBAMAZEPINE; ENVIRONMENT; INHIBITION; DEPURATION;
D O I：
10.1016/j.scitotenv.2014.05.055
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
In this study, the tissue distribution, bioconcentration, metabolism and biological effects of the macrolide antibiotic erythromycin (ERY) were investigated in fish using crucian carp (Carassius auratus) as a model. Crucian carp were exposed to various concentrations of ERY (4, 20, and 100 mu g/L) for 28 days. The UPLC/MS/MS analysis of both water and tissue provided the bioconcentration of ERY and its metabolites in the fish body. The results from tissue samples showed that a maximum tissue concentration occurred in the muscle and that the bioconcentration factor (BCF) of 72.2 was lower than the theoretical BCF of 90.4 calculated from the octanol-water coefficient of ERY. A significant portion of the absorbed ERY was metabolized via demethylation and dehydration and observed in the form of descladinose in fish. In addition, the relevant biomarkers, including acetylcholinesterase in the brain, as well as 7-ethoxyresorufin O-deethylase and superoxide dismutase in the liver, changed significantly during 28 days of exposure (P < 0.05). These results clearly indicated that ERY accumulated in fish and that similar metabolites as those observed in mammals were produced, resulting in the biochemical disturbance of biological systems. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：914 / 920
页数：7
相关论文
共 51 条
[1]   A novel method using cyanobacteria for ecotoxicity test of veterinary antimicrobial agents [J].
Ando, Tomonori ;
Nagase, Hiroyasu ;
Eguchi, Kaoru ;
Hirooka, Takashi ;
Nakamura, Takanori ;
Miyamoto, Kazuhisa ;
Hirata, Kazumasa .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2007, 26 (04) :601-606
[2]   Occurrence of bisphenol A in surface water and uptake in fish: evaluation of field measurements [J].
Belfroid, A ;
van Velzen, M ;
van der Horst, B ;
Vethaak, D .
CHEMOSPHERE, 2002, 49 (01) :97-103
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   Detection of naproxen and its metabolites in fish bile following intraperitoneal and aqueous exposure [J].
Brozinski, Jenny-Maria ;
Lahti, Marja ;
Oikari, Aimo ;
Kronberg, Leif .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2011, 18 (05) :811-818
[5]   Bioaccumulation, depuration and biotransformation of 4,4′-dibromodiphenyl ether in crucian carp (Carassius auratus) [J].
Cheng, Jie ;
Mao, Liang ;
Zhao, Zhigang ;
Shen, Mengnan ;
Zhang, Shenghu ;
Huang, Qingguo ;
Gao, Shixiang .
CHEMOSPHERE, 2012, 86 (05) :446-453
[6]   Exposure to human pharmaceuticals Carbamazepine, Ibuprofen and Bezafibrate causes molecular effects in Dreissena polymorpha [J].
Contardo-Jara, Valeska ;
Lorenz, Claudia ;
Pflugmacher, Stephan ;
Nuetzmann, Gunnar ;
Kloas, Werner ;
Wiegand, Claudia .
AQUATIC TOXICOLOGY, 2011, 105 (3-4) :428-437
[7]   INTERFERON SUPPRESSES ERYTHROMYCIN METABOLISM IN RATS AND HUMAN-SUBJECTS [J].
CRAIG, PI ;
TAPNER, M ;
FARRELL, GC .
HEPATOLOGY, 1993, 17 (02) :230-235
[8]  
EA, 2005, SC030039SR EA
[9]  
Eiser J. R., 2007, Environmental Hazards, V7, P150, DOI 10.1016/j.envhaz.2007.05.004
[10]   Proliferation of antibiotic resistance genes in microbial consortia of sequencing batch reactors (SBRs) upon exposure to trace erythromycin or erythromycin-H2O [J].
Fan, Caian ;
He, Jianzhong .
WATER RESEARCH, 2011, 45 (10) :3098-3106
123456