Rapid Determination of N-Methylcarbamoyl Adduct in Hemoglobin of Workers Exposed to N,N-Dimethylformamide by Ultra High Performance Liquid Chromatography-Mass Spectrometry

A rapid analytical method was developed for the determination of N-methylcarbamoyl adduct in Hemoglobin of workers exposed to N, N-dimethylformamide by ultra high performance liquid chromatography mass spectrometry (UPLC/MS/MS). About 0.1 g of hemoglobin sample, 40 mu mol/L of 3-methyl-5-isopropylhydantoin (MIH) as the internal standard Then the tube was heated in boiling water bath for 1h. After cooling down, 200 mu L of the mixture and 600 mu L of formic acid-acetonitrile (1%) were added into 96-well extract plate. The vacuum pump pressure was controlled to make the sample collection elute within 2-4 min.The purified collection was transferred into the sample vial, and 3-methyl-5-isopropylhydantoin (MVH) as degration product of N-methylcarbamoyl adduct was quantified by UPLC/MS/MS in multiple reaction monitoring (MRM) by internal standard method. A good linear relationship was obtained in the MVH concentration range of 0.01-1.0 mu mol/L with the correlation coefficient of 0.999. The recovery of added MVH in the blank sample was 97.3% and the relative standard deviation was 1.7%. The limit of detection (LOD) was 0.01 mu mol/g. This method was proved to be fast and efficient.