Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge

被引：16
作者：
He, Su [1 ]
Ding, Li-li [1 ]
Xu, Ke [1 ]
Geng, Jin-ju [1 ]
Ren, Hong-qiang [1 ]
机构：
[1] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 211卷
基金：
国家高技术研究发展计划(863计划);
关键词：
Low temperature; Activated sludge; Unsaturated fatty acid; High-throughput sequencing; Highly UFA biosynthesis; COMMUNITY STRUCTURE; MEMBRANE-FLUIDITY; WASTE-WATER; REMOVAL; MICROORGANISMS; GROWTH;
D O I：
10.1016/j.biortech.2016.03.069
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Low temperature is a limiting factor for the microbial activity of activated sludge for sewage treatment plant in winter. Highly unsaturated fatty acid (UFA) biosynthesis, phospholipid fatty acid (PLFA) constituents and microbial structure in activated sludge at low temperature were investigated. Over 12 gigabases of metagenomic sequence data were generated with the Illumina HiSeq 2000 platform. The result showed 43.11% of phospholipid fatty acid (PLFA) in the activated sludge participated in UFA biosynthesis, and gamma-Linolenic could be converted to Arachidonic acid at low temperature. The highly UFA biosynthesis in activated sludge was n-6 highly UFA biosynthesis, rather than n-3 highly UFA biosynthesis. The microbial community structures of activated sludge were analyzed by PLFA and high-throughput sequencing (HiSeq) simultaneously. Acidovorax, Pseudomonas, Flavobacterium and Polaromonas occupied higher percentage at 5 degrees C, and genetic changes of highly UFA biosynthesis derived from microbial community structures change. (C) 2016 Published by Elsevier Ltd.
引用
收藏
页码：494 / 501
页数：8
相关论文
共 36 条
  • [1] A metagenome of a full-scale microbial community carrying out enhanced biological phosphorus removal
    Albertsen, Mads
    Hansen, Lea Benedicte Skov
    Saunders, Aaron Marc
    Nielsen, Per Halkjaer
    Nielsen, Kare Lehmann
    [J]. ISME JOURNAL, 2012, 6 (06) : 1094 - 1106
  • [2] Burdock T., 2011, Advances in Bioscience and Biotechnology, V2, P214, DOI 10.4236/abb.2011.24032
  • [3] Chattopadhyay MK, 2001, POLAR BIOL, V24, P386
  • [4] Factors affecting the removal of organic micropollutants from wastewater in conventional treatment plants (CTP) and membrane bioreactors (MBR)
    Cirja M.
    Ivashechkin P.
    Schäffer A.
    Corvini P.F.X.
    [J]. Reviews in Environmental Science and Bio/Technology, 2008, 7 (1) : 61 - 78
  • [5] Fluorescence spectroscopy reveals ubiquitous presence of oxidized and reduced quinones in dissolved organic matter
    Cory, RM
    McKnight, DM
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2005, 39 (21) : 8142 - 8149
  • [6] Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough
    Dang, Hongyue
    Zhu, Hu
    Wang, Jing
    Li, Tiegang
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2009, 25 (01) : 71 - 79
  • [7] Influence of Anaerobiosis and Low Temperature on Bacillus cereus Growth, Metabolism, and Membrane Properties
    de Sarrau, Benoit
    Clavel, Thierry
    Clerte, Caroline
    Carlin, Frederic
    Ginies, Christian
    Christophe Nguyen-The
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (06) : 1715 - 1723
  • [8] Membrane properties of Enchytraeus albidus originating from contrasting environments: a comparative analysis
    Fisker, Karina Vincents
    Bouvrais, Helene
    Overgaard, Johannes
    Schoettner, Konrad
    Ipsen, John H.
    Holmstrup, Martin
    [J]. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 2015, 185 (04): : 389 - 400
  • [9] Microbial community structure and oxidative enzyme activity in nitrogen-amended north temperate forest soils
    Gallo, M
    Amonette, R
    Lauber, C
    Sinsabaugh, RL
    Zak, DR
    [J]. MICROBIAL ECOLOGY, 2004, 48 (02) : 218 - 229
  • [10] Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing
    Guo, Jianhua
    Peng, Yongzhen
    Ni, Bing-Jie
    Han, Xiaoyu
    Fan, Lu
    Yuan, Zhiguo
    [J]. MICROBIAL CELL FACTORIES, 2015, 14
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