Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge

被引：17
作者：
He, Su [1 ]
Ding, Li-li [1 ]
Xu, Ke [1 ]
Geng, Jin-ju [1 ]
Ren, Hong-qiang [1 ]
机构：
[1] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 211卷
基金：
国家高技术研究发展计划(863计划);
关键词：
Low temperature; Activated sludge; Unsaturated fatty acid; High-throughput sequencing; Highly UFA biosynthesis; COMMUNITY STRUCTURE; MEMBRANE-FLUIDITY; WASTE-WATER; REMOVAL; MICROORGANISMS; GROWTH;
D O I：
10.1016/j.biortech.2016.03.069
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Low temperature is a limiting factor for the microbial activity of activated sludge for sewage treatment plant in winter. Highly unsaturated fatty acid (UFA) biosynthesis, phospholipid fatty acid (PLFA) constituents and microbial structure in activated sludge at low temperature were investigated. Over 12 gigabases of metagenomic sequence data were generated with the Illumina HiSeq 2000 platform. The result showed 43.11% of phospholipid fatty acid (PLFA) in the activated sludge participated in UFA biosynthesis, and gamma-Linolenic could be converted to Arachidonic acid at low temperature. The highly UFA biosynthesis in activated sludge was n-6 highly UFA biosynthesis, rather than n-3 highly UFA biosynthesis. The microbial community structures of activated sludge were analyzed by PLFA and high-throughput sequencing (HiSeq) simultaneously. Acidovorax, Pseudomonas, Flavobacterium and Polaromonas occupied higher percentage at 5 degrees C, and genetic changes of highly UFA biosynthesis derived from microbial community structures change. (C) 2016 Published by Elsevier Ltd.
引用
收藏
页码：494 / 501
页数：8
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