Improved Viability of Microencapsulated Probiotics in a Freeze-Dried Banana Powder During Storage and Under Simulated Gastrointestinal Tract

被引:46
作者
Bora, Awa Fanny Massounga [1 ,2 ]
Li, Xiaodong [1 ,2 ]
Zhu, Yongming [1 ,2 ]
Du, Lingling [1 ,2 ]
机构
[1] Northeast Agr Univ, Coll Food Sci, 600 Changjiang St, Harbin 150030, Heilongjiang, Peoples R China
[2] Northeast Agr Univ, Key Lab Dairy Sci, Minist Educ, 600 Changjiang St, Harbin 150030, Heilongjiang, Peoples R China
关键词
Microencapsulation; Probiotics; Whey protein isolate; Fructooligosaccharides; Freeze-dried banana powder; Viability; LACTIC-ACID BACTERIA; WHEY-PROTEIN; LACTOBACILLUS-ACIDOPHILUS; GASTRIC-JUICE; SURVIVAL; BIFIDOBACTERIA;
D O I
10.1007/s12602-018-9464-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Freeze-dried banana powder represents an ideal source of nutrients and has not yet been used for probiotic incorporation. In this study, microencapsulation by freeze drying of probiotics Lactobacillus acidophilus and Lactobacillus casei was made using whey protein isolate (WPI), fructooligosaccharides (FOS), and their combination (WPI + FOS) at ratio (1:1). Higher encapsulation yield was found for (WPI + FOS) microspheres (98%). Further, microcapsules of (WPI + FOS) were used to produce a freeze-dried banana powder which was analyzed for bacterial viability under simulated gastrointestinal fluid (SGIF), stability during storage at 4 degrees C and 25 degrees C, and chemical and sensory properties. Results revealed that (WPI + FOS) microcapsules significantly increased bacteria stability in the product over 30 days of storage at 4 degrees C averaging (>= 8.57 log CFU/g) for L. acidophilus and (>= 7.61 log CFU/g) for L. Casei as compared to free cells. Bacteria encapsulated in microspheres (WPI + FOS) were not significantly affected by the SGIF, remaining stable up to 7.05 +/- 0.1 log CFU/g for L.acidophilus and 5.48 +/- 0.1 log CFU/g for L.casei after 90 min of incubation at pH 2 compared to free cells which showed minimal survival. Overall, encapsulated probiotics enriched freeze-dried banana powders received good sensory scores; they can therefore serve as safe probiotics food carriers.
引用
收藏
页码:1330 / 1339
页数:10
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