Role of PI3K/Akt signaling in the protective effect of magnesium sulfate against ischemia-perfusion injury of small intestine in rats

被引：15
作者：
Chen Shi-da [1 ]
Chen Ya-bin [1 ]
Peng You [1 ]
Xu Jia [1 ]
Chen Su-shan [1 ]
Zhang Jun-long [1 ]
Li Zheng-zhang [1 ]
Tan Zhi [1 ]
机构：
[1] Sun Yat Sen Univ, Dept Physiol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
来源：
CHINESE MEDICAL JOURNAL | 2010年 / 123卷 / 11期
关键词：
PI3K/Akt signal pathway; magnesium sulfate; reperfusion; apoptosis; oxidative stress; intestinal mucosa; PHOSPHOINOSITIDE 3-KINASE PATHWAY; REPERFUSION INJURY; ARTERY-OCCLUSION; CELL-DEATH; IN-VIVO; APOPTOSIS; ACTIVATION; TRANSDUCTION; GLUTAMINE; SEPSIS;
D O I：
10.3760/cma.j.issn.0366-6999.2010.11.018
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background The protective effects of magnesium sulfate against ischemia-reperfusion injury of the small intestine in Sprague-Dawley (SD) rats have been confirmed in our previous research. However, its exact mechanism is unclear. This study was to evaluate the role of PI3K/Akt signal pathway in the protective effect of magnesium sulfate against ischemia-reperfusion injury of the small intestine in SD rats. Methods Rat model of intestinal ischemia-reperfusion injury was used. The SD rats were divided into four groups randomly: sham operation group, ischemia-reperfusion group, magnesium sulfate group and magnesium sulfate plus LY294002 (an inhibitor of PI3K) group. The pathological changes of intestinal mucosa were examined; the activity of diamine oxidase (DAO) in plasma, the plasma contents of malondialdehyde (MDA), and apoptosis rate of the intestinal mucosal cells were determined and compared. The expression of p-Akt was detected by Western blotting. Results There were more evident pathological changes of the intestinal mucosa (higher Chiu's score, P <0.05), enhanced DAO activity (P <0.05), elevated contents of MDA (P <0.05), higher apoptosis rate (P <0.05), and lower level of p-Akt (P <0.05) in the ischemia-reperfusion group compared with the sham operation group. There were less evident pathological changes of the intestinal mucosa (lower Chiu's score, P <0.05), lower DAO activity (P <0.05), lower contents of MDA (P <0.05), and lower apoptosis rate (P <0.05), but higher level of p-Akt (P <0.05) in the magnesium sulfate group compared with the ischemia-reperfusion group. There were more evident pathological changes of the intestinal mucosa (higher Chiu's score, P <0.05), higher contents of MDA (P <0.05), higher DAO activity (P <0.05) and higher apoptosis rate (P <0.05), and lower level of p-Akt (P <0.05) in the magnesium sulfate plus LY294002 group compared with the magnesium sulfate group. Conclusions Activation of PI3K/Akt signal pathway results in the reduction of cell apoptosis, which likely accounts for the protective effect of magnesium sulfate against intestinal ischemia-reperfusion injury. Chin Med J 2010;123(11):1447-1452
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页码：1447 / 1452
页数：6
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