Curcumin suppresses α-melanocyte stimulating hormone-stimulated melanogenesis in B16F10 cells

被引：46

作者：

Lee, Jun-Hyuk ^{[2
]}

Jang, Ji-Yeon ^{[1
]}

Park, Cheol ^{[3
,5
]}

Kim, Byung-Woo ^{[4
,5
]}

Choi, Yung-Hyun ^{[3
,5
]}

Choi, Byung-Tae ^{[1
,5
]}

机构：

[1] Pusan Natl Univ, Sch Korean Med, Div Meridian & Struct Med, Gyeongnam 626870, South Korea

[2] Chem & Biotechnol Examinat Bur, Korean Intellectual Property Off, Taejon 302701, South Korea

[3] Dong Eui Univ, Coll Oriental Med, Dept Biochem, Pusan 614052, South Korea

[4] Dong Eui Univ, Coll Nat Sci, Dept Life Sci & Biotechnol, Pusan 614714, South Korea

[5] Dong Eui Univ, Blue Bio Ind RIC, Pusan 614714, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2010年 / 26卷 / 01期

关键词：

curcumin; melanogenesis; tyrosinase; microphthalmia-associated transcription factor; extracellular signal-regulated kinase; Akt; SIGNAL-REGULATED KINASE; PROTEIN-KINASE; PHOSPHATIDYLINOSITOL-3-KINASE PATHWAY; INHIBITS MELANOGENESIS; KAPPA-B; ACTIVATION; TRANSCRIPTION; INVOLVEMENT; TARGETS;

D O I：

10.3892/ijmm_00000440

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study was designed to assess the potential inhibitory activity of curcumin on the a-melanocyte stimulating hormone (alpha-MSH)-stimulated melanogenesis signal pathway in B16F10 melanoma cells. The molecular mechanism of curcumin-induced inhibitory activity on the alpha-MSH-stimulated melanogenesis signal pathway, including expression of melanogenesis-related proteins and activation of melanogenesis-regulating proteins, was examined in B16F10 cells. Curcumin suppressed the cellular melanin contents and the tyrosinase activity in alpha-MSH-stimulated B16F10 cells. In addition, the expression of melanogenesis-related proteins such as microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein 1 and 2 was suppressed by curcumin in the alpha-MSH-stimulated B16F10 cells. Notably, a melanogenesis-regulating signal such as mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) or phosphatidylinositol 3-kinase (PI3K)/Akt was activated by curcumin in the B16F10 cells treated with or without alpha-MSH. The suppressive activity of curcumin on alpha-MSH-induced melanogenesis was down-regulated by PD98059 and by LY294002. Our results suggest that the suppressive activity of curcumin on alpha-MSH-stimulated melanogenesis may involve the down-regulation of MITF and its downstream signal pathway through the activation of MEK/ERK or PI3K/Akt.

引用

页码：101 / 106

页数：6

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