Transcriptomic and epigenetic analysis of breast cancer stem cells

被引：22
作者：
Li, Guochao [1 ,2 ]
Wang, Dong [1 ,2 ]
Ma, Wencui [3 ]
An, Ke [1 ,2 ]
Liu, Zongzhi [1 ,2 ]
Wang, Xinyu [4 ]
Yang, Caiyun [1 ,2 ]
Du, Fengxia [1 ,2 ]
Han, Xiao [1 ,2 ]
Chang, Shuang [1 ,2 ]
Yu, Hui [1 ,2 ]
Zhang, Zilong [1 ,2 ]
Zhao, Zitong [1 ,2 ]
Zhang, Yan
Wang, Junyun [1 ,2 ]
Sun, Yingli [1 ,2 ]
机构：
[1] Chinese Acad Sci, Beijing Inst Genom, China Gastrointestinal Canc Res Ctr, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Heze Third Peoples Hosp, Qingdao 274031, Shandong, Peoples R China
[4] Harbin Med Univ, Coll Bioinformat Sci & Technol, Harbin 150081, Heilongjiang, Peoples R China
来源：
EPIGENOMICS | 2018年 / 10卷 / 06期
基金：
中国国家自然科学基金;
关键词：
breast cancer stem cells; DNA methylation; epigenome; histone modification; H3K27me3; H3K4me2; multiple omics analysis; transcriptome; TRANSLATION INITIATION; SIGNALING PATHWAY; READ ALIGNMENT; SELF-RENEWAL; EXPRESSION; GROWTH; EIF4E; METHYLATION; METASTASIS; CARCINOMA;
D O I：
10.2217/epi-2018-0008
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: Cancer stem cells (CSCs) drive triple-negative breast cancer recurrence via their properties of self-renewal, invasiveness and radio/chemotherapy resistance. This study examined how CSCs might sustain these properties. Materials & methods: Transcriptomes, DNA methylomes and histone modifications were compared between CSCs and non CSCs. Results: Transcriptome analysis revealed several pathways that were activated in CSCs, whereas cell cycle regulation pathways were inhibited. Cell development and signaling genes were differentially methylated, with histone methylation analysis suggesting distinct H3K4me2 and H3K27me3 enrichment profiles. An integrated analysis revealed several tumor suppressor genes downregulated in CSCs. Conclusion: Differential activation of various signaling pathways and genes contributes to the tumor-promoting properties of CSCs. Therapeutic targets identified in the analysis may contribute to improving treatment options for patients.
引用
收藏
页码：765 / 783
页数：19
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