Assessing the immunomodulatory potential of high-molecular-weight extracts from mushrooms; an assay based on THP-1 macrophages

被引:5
作者
van de Velde, Jan [1 ]
Wilbers, Ruud H. P. [1 ]
Westerhof, Lotte B. [1 ]
van Raaij, Debbie R. [1 ]
Stavrakaki, Ioanna [1 ]
Sonnenberg, Anton S. M. [1 ]
Bakker, Jaap [1 ]
Schots, Arjen [1 ]
机构
[1] Univ Wageningen & Res Ctr, Plant Sci Grp, Wageningen, Netherlands
关键词
immunomodulation; cytokines; macrophages; cell-based assay; THP-1; cells; bioactive compounds; MONOCYTIC LEUKEMIA-CELLS; AGARICUS-BLAZEI MURILL; TOLL-LIKE RECEPTORS; INDUCTION; POLYSACCHARIDES; DIFFERENTIATION; EXPRESSION; RESPONSES; GLUCANS; DISEASE;
D O I
10.1002/jsfa.6726
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
BACKGROUNDFood is a potential source of immunomodulating compounds that may be used to steer immune responses towards a desired status such as reducing inflammatory disorders. However, to identify and characterize such bioactive compounds, biologically relevant and standardized assays are required. Macrophages play an important role in immunomodulation and are suited for developing cell-based assays. An assay was developed based on macrophages, in a homogeneous differentiation state, using the human monocytic cell line THP-1 previously used to assess immunomodulatory properties of low-molecular-weight allergens, hormones, dietary supplements and therapeutic drugs. RESULTSZymosan and mushroom polysaccharide extracts lead to a heterogeneous differentiation state of THP-1 monocytes, and these cells secrete low levels of cytokines upon stimulation. Differentiation into macrophages using a low concentration of phorbol 12-myristate 13-acetate improved responsiveness. Elevated levels of cytokines were secreted by cells in a homogenous differentiation state. In addition, it was determined that the assay performs best when using cells at a concentration of (2.5-5) x 10(5) cells mL(-1). CONCLUSIONAn assay was developed suitable to distinguish the immunomodulatory properties of food compounds in a reproducible manner. It was evaluated using eight mushroom species by measuring the secretion of relevant cytokines TNF-, IL-1, IL-6 and IL-10. (c) 2014 Society of Chemical Industry
引用
收藏
页码:344 / 350
页数:7
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