Expression of fibronectin binding protein A (FnBPA) from Staphylococcus aureus at the cell surface of Lactococcus lactis improves its immunomodulatory properties when used as protein delivery vector

被引：17

作者：

Almeida, Juliana F. ^{[1
,2
]}

Breyner, Natalia M. ^{[1
,2
]}

Mahi, Miloud ^{[1
,3
]}

Ahmed, Bensoltane ^{[3
]}

Benbouziane, Bouasria ^{[1
,4
]}

Boas, Priscilla C. B. Vilas ^{[1
,2
]}

Miyoshi, Anderson ^{[2
]}

Azevedo, Vasco ^{[2
]}

Langella, Philippe ^{[1
]}

Bermudez-Humaran, Luis G. ^{[1
]}

Chatel, Jean-Marc ^{[1
]}

机构：

[1] Univ Paris Saclay, AgroParisTech, INRA, Micalis Inst, F-78350 Jouy En Josas, France

[2] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil

[3] Univ Nat Life & Hearth Sci, Food Microbiol Lab, Dept Biol, Theniat El Had St, Khemis Miliana 44225, Algeria

[4] Univ Mostaganem, Dept Biol, Lab Beneficial Microorganisms Funct Foods & Hlth, Lahcen St,POB 300, Mostaganem 27000, Algeria

来源：

VACCINE | 2016年 / 34卷 / 10期

关键词：

Lactic acid bacteria; Protein vaccination; Fibronectin binding protein A (FnBPA); Human papillomavirus (HPV); HPV-16-E7; antigen; EPITHELIAL-CELLS; DENDRITIC CELLS; ACID BACTERIA; TYPE-16; E7; IN-VIVO; MONOCYTOGENES INTERNALIN; MUCOSAL DELIVERY; IMMUNIZATION; ANTIGEN; DNA;

D O I：

10.1016/j.vaccine.2016.01.022

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A recombinant strain of Lactococcus lactis displaying a cell-surface anchored fibronectin binding protein A (FnBPA) from Staphylococcus aureus (LL-FnBPA) had been shown to be more efficient in delivering plasmid than its wild-type counterpart both in vitro and in vivo, and have the ability to orientate the immune response toward a Th2 profile in a context of a DNA vaccination. The aim of this work was to test whether this LL-FnBPA strain could shape the immune response after mucosal administration in mice. For this, we used a mouse model of human papilloma virus (HPV)-induced cancer and a L. lactis strain displaying at its cell surface both HPV-16-E7 antigen (LL-E7) and FnBPA (LL-E7 + FnBPA). Our results revealed a more efficient systemic Th1 immune response with recombinant LL-E7 + FnBPA. Furthermore, mice vaccinated with LL-E7 + FnBPA were better protected when challenged with HPV-16-induced tumors. Altogether, the results suggest that FnBPA displays adjuvant properties when used in the context of mucosal delivery using L. lactis as a live vector. (C) 2016 Published by Elsevier Ltd.

引用

页码：1312 / 1318

页数：7

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