Microencapsulated chlorpyrifos: Degradation in soil and influence on soil microbial community structures

Degradation kinetics of microencapsulated chlorpyrifos (CPF-MC) in soil and its influence on soil microbial community structures were investigated by comparing with emulsifiable concentration of chlorpyrifos (CPF-EC) in laboratory. The residual periods of CPF-MC with fortification levels of 5 and 20 mg/kg reached 120 days in soil, both of the degradation curves did not fit the first-order model, and out-capsule residues of chlorpyrifos in soil were maintained at 1.76 (+/- 0.33) and 5.92 (+/- 1.20) mg/kg in the period between 15 and 60 days, respectively. The degradation kinetics of CPF-EC fit the first-order model, and the residual periods of 5 and 20 mg/kg treatments were 60 days. Bacterial community structures in soil treated with two concentrations of CPF-MC showed similarity to those of the control during the test period, as seen in the band number and relative intensities of the individual band on DGGE gels (p > 0.05). Fungal community structures were slightly affected in the 5 mg/kg treatments and returned to the control levels after 30 days, but initially differed significantly from control in the 20 mg/kg treatments (p < 0.05) and did not recover to control levels until 90 days later. The CPF-EC significantly altered microbial community structures (p < 0.05) and effects did not disappear until 240 days later. The results indicated that the microcapsule technology prolonged the residue periods of chlorpyrifos in soil whereas it decreased its side-effects on soil microbes as compared with the emulsifiable concentration formulation. (C) 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.