trans activity of the norovirus Camberwell proteinase and cleavage of the N-terminal protein encoded by ORF1

被引:17
作者
Seah, EL [1 ]
Marshall, JA
Wright, PJ
机构
[1] Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia
[2] Victorian Infect Dis Reference Lab, Melbourne, Vic 3051, Australia
关键词
D O I
10.1128/JVI.77.12.7150-7155.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The virus-encoded proteinase of Camberwell virus, a genogroup 2 norovirus, was synthesized in Escherichia coli. The purified proteinase had correct N and C termini and showed trans activity in cell-free assays. trans activity was also demonstrated in COS cells transfected with constructs encoding either the proteinase or a proteinase-polymerase fusion. The N-terminal protein of ORF1 was cleaved in COS cells, possibly at the site E-194/S.
引用
收藏
页码:7150 / 7155
页数:6
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