Pro- and anti-inflammatory cytokine expression in immune organs of the common carp exposed to atrazine and chlorpyrifos

被引：51
作者：
Chen, Dechun [1 ,2 ]
Zhang, Ziwei [1 ]
Yao, Haidong [1 ]
Cao, Ye [1 ]
Xing, Houjuan [1 ,3 ]
Xu, Shiwen [1 ]
机构：
[1] Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China
[2] Jilin Engn Vocat Coll, Dept Biol Engn, Siping 136001, Peoples R China
[3] Anim Hlth Supervis Inst Heilonijiang Prov, Harbin 150069, Peoples R China
来源：
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2014年 / 114卷
基金：
中国博士后科学基金;
关键词：
Common carp; Cytokine; Immune organ; Atrazine; Chlorpyrifos; TROUT ONCORHYNCHUS-MYKISS; NECROSIS-FACTOR-ALPHA; INFLAMMATORY RESPONSE; MOLECULAR-CLONING; OXIDATIVE STRESS; TNF-ALPHA; IN-VITRO; BLOOD; RATS; MICE;
D O I：
10.1016/j.pestbp.2014.07.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Atrazine (ATR) and chlorpyrifos (CPF) are toxic and subject to long-term in vivo accumulation in different aquatic species throughout the world. The purpose of the present study was to examine the effect of ATR, CPF and combined ATR/CPF exposure on cytokines in the head kidney and spleen of common carp (Cyprinus carpio L.). The carp were sampled after a 40-d exposure to CPF and ATR, individually or in combination, followed by a 40-d recovery to measure the mRNA expression of IL-6fam (IL-6), IL-8, TNF-alpha, IL-10 and TGF-beta 1 (TGF-beta) in the head kidney and spleen tissues. These results showed that the expression of cytokines IL-6, IL-8 and TNF-alpha in the head kidney and spleen was upregulated following ATR, CPF and mixed ATR/CPF exposure compared with the control group. The expression of IL-10 and TGF-beta mRNA was significantly inhibited in both head kidney and spleen of carp exposed to AIR, CPF and the ATR/CPF mixture. The results suggested that long-term exposure of ATR, CPF and the ATR/CPF mixture in aquatic environments can induce the dysregulation of pro-/anti-inflammatory cytokine expression. The information regarding the effects of ATR and CPF on cytokine mRNA expression generated in this study will be important information for pesticides toxicology evaluation. (C) 2014 Elsevier Inc. All rights reserved.
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页码：8 / 15
页数：8
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