Giant magneto-resistance based immunoassay for the tumor marker carcinoembryonic antigen

被引：15
作者：
Sun, Xue-cheng [1 ]
Lei, Chong [1 ]
Guo, Lei [1 ]
Zhou, Yong [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Minist Educ,Dept Micronano Elect, Key Lab Thin Film & Microfabricat, Dong Chuan Rd 800, Shanghai 200240, Peoples R China
来源：
MICROCHIMICA ACTA | 2016年 / 183卷 / 03期
基金：
上海市自然科学基金; 中国国家自然科学基金;
关键词：
Magnetic particles; MEMS; Gold film; Lithography; Sputtering; MAGNETICALLY LABELED BIOMOLECULES; SPIN-VALVE SENSORS; GOLD NANOPARTICLES; DNA DETECTION; IMMUNOSENSOR; BIOSENSOR; BEADS; MICROBEADS; SEPARATION; ELECTRODE;
D O I：
10.1007/s00604-015-1686-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We report on a giant magnetoresistance (GMR) based immunosensor for the carcinoembryonic antigen (CEA) labeled with superparamagnetic microparticles (Dynabeads). The GMR sensor contains 200 GMR strips and was fabricated by micro-electromechanical system (MEMS) technology. This system can detect Dynabeads in concentrations down to 10 nga <...mL(-1). Sandwich immunoassays were employed on the surface of a gold film modified with a self-assembled monolayer and using biotinylated secondary antibody against CEA and streptavidinylated Dynabeads. With DC magnetic fields in the range of 40 to 90 Oe, CEA can be detected with a detection limit as low as 10 pga <...mL(-1). Samples spiked with different concentrations of CEA can be distinguished clearly, and the method is deemed to be well suited for clinical use.
引用
收藏
页码：1107 / 1114
页数：8
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