A simplified method for sample collection and DNA isolation for polymerase chain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors

被引：12

作者：

Machado, EMM

Alvarenga, NJ

Romanha, AJ

Grisard, EC

机构：

[1] Univ Fed Santa Catarina, Dept Microbiol & Parasitol, BR-88040900 Florianopolis, SC, Brazil

[2] Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Triatomineos & Epidemiol Doenca Chagas, BR-30190 Belo Horizonte, MG, Brazil

[3] Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Parasitol Celular & Mol, BR-30190 Belo Horizonte, MG, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2000年 / 95卷 / 06期

关键词：

Trypanosoma cruz; Trypanosoma rangeli; polymerase chain reaction; triatomines; Dipetalogaster maximus;

D O I：

10.1590/S0074-02762000000600021

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America, sharing several reservoirs and triatomine vectors, we herein describe a simple method to collect triatomine feces and hemolymph in filter paper for further detection and specific characterization of these two trypanosomes. Experimentally, infected triatomines feces and hemolymph were collected in filter paper and specific detection of T. rangeli or T. cruzi DNA by polymerase chain reaction was achieved. This simple DNA collection method allows sample collection in the field and further specific trypanosome detection and characterization in the laboratory.

引用

页码：863 / 866

页数：4

共 16 条

[1]

BRENER Z, 1963, Rev Inst Med Trop Sao Paulo, V5, P220

[2] FIELD APPLICATION OF POLYMERASE CHAIN-REACTION DIAGNOSIS AND STRAIN TYPING OF TRYPANOSOMA-CRUZI IN BOLIVIAN TRIATOMINES [J].