Shear stress regulation of nitric oxide production in uterine and placental artery endothelial cells: experimental studies and hemodynamic models of shear stresses on endothelial cells

被引:42
作者
Sprague, Benjamin [1 ,2 ]
Chesler, Naomi C. [2 ]
Magness, Ronald R. [1 ,3 ,4 ]
机构
[1] Univ Wisconsin, Perinatal Res Labs, Dept Obstet & Gynecol, Madison, WI 53715 USA
[2] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53715 USA
[3] Univ Wisconsin, Dept Pediat, Madison, WI 53715 USA
[4] Univ Wisconsin, Dept Anim Sci, Madison, WI 53715 USA
关键词
pregnancy; shear stress; nitric oxide; blood flow; rheology; VASODILATOR PRODUCTION; SYSTEMIC ARTERIES; SYNTHASE EXPRESSION; BLOOD-FLOW; OVINE UTERINE; PREGNANCY; CALCIUM; PHOSPHORYLATION; INHIBITION; MECHANISMS;
D O I
10.1387/ijdb.082832bs
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hemodynamic shear stress is the most powerful physiological regulator of endothelial Nitric Oxide Synthase (eNOS), leading to rapid rises in nitric oxide (NO). The substantial increases in uterine and placental blood flows throughout gestation rely heavily on the action of NO. We and others have investigated endothelial function in response to shear stress with cell culture models of shear stress. In order to apply the results of these studies more effectively, we need a more complete understanding of the origin and coupling of the hemodynamic forces and vascular tissue behavior. For example, equations commonly used to calculate in vivo shear stress incorporate assumptions of steady (non-pulsatile) blood flow and constant viscosity of blood (Newtonian fluid). Using computational models, we can estimate a waveform of shear stress over a cardiac cycle and the change in blood viscosity with shear rate and hematocrit levels, two variables that often change with size of vessel and location within a vascular tree. This review discusses hemodynamics as they apply to blood flow in vessels, in the hope that an integration of these fields can lead to improved in vitro shear stress experiments and understanding of NO production in uterine and placental vascular physiology during gestation.
引用
收藏
页码:331 / 339
页数:9
相关论文
共 57 条
[31]  
Magness RR, 1998, CONT ENDOCRINOL, V9, P507
[32]   Endothelial vasodilator production by uterine and systemic arteries .2. Pregnancy effects on NO synthase expression [J].
Magness, RR ;
Shaw, CE ;
Phernetton, TM ;
Zheng, J ;
Bird, IM .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1997, 272 (04) :H1730-H1740
[33]   Endothelial vasodilator production by uterine and systemic arteries .1. Effects of ANG II on PGI(2) and NO in pregnancy [J].
Magness, RR ;
Rosenfeld, CR ;
Hassan, A ;
Shaul, PW .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1996, 270 (06) :H1914-H1923
[34]   Endothelial vasodilator production by uterine and systemic arteries.: VI.: Ovarian and pregnancy effects on eNOS and NOx [J].
Magness, RR ;
Sullivan, JA ;
Li, Y ;
Phernetton, TM ;
Bird, IM .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2001, 280 (04) :H1692-H1698
[35]   Systemic and uterine blood flow distribution during prolonged infusion of 17β-estradiol [J].
Magness, RR ;
Phernetton, TM ;
Zheng, J .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1998, 275 (03) :H731-H743
[36]  
Magness RR ZJ., 1996, Maternal Cardiovascular Alterations During Pregnancy, V2nd
[37]   Biosynthesis and action of nitric oxide in mammalian cells [J].
Mayer, B ;
Hemmens, B .
TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (12) :477-481
[38]   DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells [J].
McCormick, SM ;
Eskin, SG ;
McIntire, LV ;
Teng, CL ;
Lu, CM ;
Russell, CG ;
Chittur, KK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (16) :8955-8960
[39]   Analysis of blood flow in the entire coronary arterial tree [J].
Mittal, N ;
Zhou, Y ;
Linares, C ;
Ung, S ;
Kaimovitz, B ;
Molloi, S ;
Kassab, GS .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2005, 289 (01) :H439-H446
[40]   N-OMEGA-NITRO-L-ARGININE, AN INHIBITOR OF NITRIC-OXIDE SYNTHESIS, INCREASES BLOOD-PRESSURE IN RATS AND REVERSES THE PREGNANCY-INDUCED REFRACTORINESS TO VASOPRESSOR AGENTS [J].
MOLNAR, M ;
HERTELENDY, F .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1992, 166 (05) :1560-1567