Substance P selectively activates TNF-alpha mRNA in rat uterine immune cells: a neuroimmune link

被引：21

作者：

Cocchiara, R

Bongiovanni, A

Albeggiani, G

Azzolina, A

Geraci, D

机构：

[1] Istituto di Biologia dello Sviluppo, CNR, 90145 Palermo

来源：

NEUROREPORT | 1997年 / 8卷 / 13期

关键词：

competitive PCR; macrophages; mast cell; neuro-immune axis; reproductive behaviour; substance P; TNF-alpha mRNA;

D O I：

10.1097/00001756-199709080-00031

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

SUBSTANCE P (SP) is a neuropeptide which influences the interaction between the nervous and immune systems. It is an important modulator of cytokines, including tumour necrosis factor-alpha (TNF-alpha) whose role during the reproductive processes has been established. We have investigated the effects of SP on TNF-alpha mRNA expression in macrophages and mast cells (MC) isolated from rat peritoneum and uterus. Cell supernatants were analysed for their histamine content as a measure of stimulation. SP alone increased TNF-alpha expression in peritoneal MC but not in peritoneal macrophages. The addition of SP resulted in a six-fold enhancement of TNF-alpha expression in uterine MC whereas no stimulation was observed in macrophages as determined by competitive polymerase chain reaction (PCR).

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收藏

页码：2961 / 2964

页数：4

相关论文

共 23 条

[1] PSYCHONEUROIMMUNOLOGY - INTERACTIONS BETWEEN THE NERVOUS-SYSTEM AND THE IMMUNE-SYSTEM [J].

ADER, R ;

COHEN, N ;

FELTEN, D .

LANCET, 1995, 345 (8942) :99-103

[2]

ANSEL JC, 1993, J IMMUNOL, V150, P4478

[3] STRESS-INDUCED MURINE ABORTION ASSOCIATED WITH SUBSTANCE BETA-DEPENDENT ALTERATION IN CYTOKINES IN MATERNAL UTERINE DECIDUA [J].

ARCK, PC ;

MERALI, FS ;

STANISZ, AM ;

STEAD, RH ;

CHAOUAT, G ;

MANUEL, J ;

CLARK, DA .

BIOLOGY OF REPRODUCTION, 1995, 53 (04) :814-819

[4]

BERMAN AS, 1996, NEUROIMMUNOMODULAT, V3, P41

[5] SYMPATHETIC-NERVE CONTACT ALTERS MEMBRANE RESISTANCE OF CELLS OF THE RBL-2H3 MUCOSAL MAST-CELL LINE [J].

BLENNERHASSETT, MG ;

JANISZEWSKI, J ;

BIENENSTOCK, J .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1992, 6 (05) :504-509

[6] CACHECTIN TUMOR NECROSIS FACTOR-ALPHA FORMATION IN HUMAN DECIDUA - POTENTIAL ROLE OF CYTOKINES IN INFECTION-INDUCED PRETERM LABOR [J].

CASEY, ML ;

COX, SM ;

BEUTLER, B ;

MILEWICH, L ;

MACDONALD, PC .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (02) :430-436

[7] MODULATION OF RAT PERITONEAL MAST-CELL AND HUMAN BASOPHIL HISTAMINE-RELEASE BY ESTROGENS [J].

COCCHIARA, R ;

ALBEGGIANI, G ;

DITRAPANI, G ;

AZZOLINA, A ;

LAMPIASI, N ;

RIZZO, F ;

GERACI, D .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1990, 93 (2-3) :192-197

[8] EFFECT OF SUBSTANCE-P ON UTERINE MAST-CELL CYTOKINE RELEASE DURING THE REPRODUCTIVE-CYCLE [J].

COCCHIARA, R ;

ALBEGGIANI, G ;

AZZOLINA, A ;

BONGIOVANNI, A ;

LAMPIASI, N ;

DIBLASI, F ;

GERACI, D .

JOURNAL OF NEUROIMMUNOLOGY, 1995, 60 (1-2) :107-115

[9] Inhibitory effect of neuraminidase on SP-induced histamine release and TNF-alpha mRNA in rat mast cells: Evidence of a receptor-independent mechanism [J].

Cocchiara, R ;

Bongiovanni, A ;

Albeggiani, G ;

Azzolina, A ;

Lampiasi, N ;

DiBlasi, F ;

Geraci, D .

JOURNAL OF NEUROIMMUNOLOGY, 1997, 75 (1-2) :9-18

[10] A factor secreted by human embryo stimulates cytokine release by uterine mast cell [J].

Cocchiara, Roberta ;

Lampiasi, Nadia ;

Albeggiani, Giuseppe ;

Azzolina, Antonina ;

Bongiovanni, Antonella ;

Gianaroli, Luca ;

Di Blasi, Francesco ;

Geraci, Domenico .

MOLECULAR HUMAN REPRODUCTION, 1996, 2 (10) :781-791