Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae

被引：3

作者：

Cheng, B. B. ^{[1
]}

Sun, Q. W. ^{[1
]}

Zheng, Y. Q. ^{[1
]}

机构：

[1] Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture State Forestry Adm, Lab Mol Identificat Plant Varieties,Res Inst Fore, Beijing, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 04期

关键词：

Taxus wallichiana var. wallichiana; Microsatellite loci; Cross-amplification; Genetic structure; ARRAYS;

D O I：

10.4238/2015.December.7.15

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross- amplification of the 9 loci was observed in 2 other varieties of T. wallichiana and 4 other Taxaceae species. These loci will be of value for studying population genetic structures and for genetic resource conservation in T. wallichiana and other Taxus species.

引用

页码：16018 / 16023

页数：6

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