miR-29b affects neurocyte apoptosis by targeting MCL-1 during cerebral ischemia/reperfusion injury

被引:24
作者
Huang, Zhi [1 ,2 ]
Lu, Lu [3 ]
Jiang, Tianpeng [2 ]
Zhang, Shuai [2 ]
Shen, Yaping [1 ]
Zheng, Zhu [4 ]
Zhao, Ansu [4 ]
Gao, Rui [5 ]
Li, Rui [6 ]
Zhou, Shi [1 ,2 ]
Liu, Jing [2 ]
机构
[1] Guizhou Med Univ, Affiliated Baiyun Hosp, Dept Intervent, 28 Guiyi Rd, Guiyang 550002, Guizhou, Peoples R China
[2] Guizhou Med Univ, Affiliated Hosp, Dept Intervent, 28 Guiyi Rd, Guiyang 550002, Guizhou, Peoples R China
[3] Shenzhen Eye Hosp, Shenzhen Key Lab Ophthalmoloy, Shenzhen 518040, Guangdong, Peoples R China
[4] Guizhou Med Univ, Sch Biol & Engn, Guiyang 550001, Guizhou, Peoples R China
[5] Guizhou Entry Exit Inspect & Quarantine Bur Peopl, Guiyang 550002, Guizhou, Peoples R China
[6] Guizhou Prov Peoples Hosp, Dept Rehabil, Guiyang 550002, Guizhou, Peoples R China
关键词
miR-29b; MCL-1; cerebral ischemia-reperfusion injury; neural apoptosis; ISCHEMIC-STROKE; EXPRESSION; CELLS;
D O I
10.3892/etm.2018.6622
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The present study aimed to determine whether an miRNA (miR)-29b inhibitor protected against cerebral ischemia/reperfusion (I/R) injury in vitro and to investigate the underlying mechanisms. As a model for induced cerebral IR injury, N2a cells were exposed to an oxygen-glucose deprivation/reoxygenation (OGD/R) environment. Using this model, it was demonstrated that miR-29b was significantly upregulated compared with cells in a normal environment. The interactions between miR-29b and myeloid cell leukemia sequence (MCL)-1 were then investigated using dual-luciferase assays, revealing a strong regulation of MCL-1 through the 3untranslated region. Using the OGD/R model, the present study additionally examined the effects of miR-29b and miR-29b inhibitor on cell viability and apoptosis using Cell Counting kit 8 and flow cytometry assays, respectively. miR-29b transfection led to increased N2a cell apoptosis and reduced cell viability under an OGD/R environment. However, this effect was reversed by the miR-29b inhibitor. Finally, the effects of miR-29b on the expression of several Wnt-associating proteins were examined. It was observed that B cell lymphoma-2 was inhibited by miR-29b, as was MCL-1, whereas caspase-3 expression was promoted. The miR-29b inhibitor demonstrated the opposite effect. Overall, miR-29b promoted neurocyte apoptosis by targeting MCL-1 during cerebral I/R injury. The results of the present study suggest a potential novel therapeutic target for the treatment of ischemic stroke.
引用
收藏
页码:3399 / 3404
页数:6
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