The transcriptome of mouse central nervous system myelin

被引：59

作者：

Thakurela, Sudhir ^{[1
]}

Garding, Angela ^{[1
]}

Jung, Ramona B. ^{[2
]}

Mueller, Christina ^{[3
]}

Goebbels, Sandra ^{[2
]}

White, Robin ^{[3
]}

Werner, Hauke B. ^{[2
]}

Tiwari, Vijay K. ^{[1
]}

机构：

[1] Inst Mol Biol, D-55128 Mainz, Germany

[2] Max Planck Inst Expt Med, Dept Neurogenet, Hermann Rein Str 3, D-37075 Gottingen, Germany

[3] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Inst Physiol, D-55128 Mainz, Germany

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

欧洲研究理事会;

关键词：

BASIC-PROTEIN GENE; MESSENGER-RNA; PROTEOLIPID PROTEIN; OLIGODENDROCYTES; EXPRESSION; TRANSPORT; MUTANT; GLIA; JUNCTIONS; MEMBRANE;

D O I：

10.1038/srep25828

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rapid nerve conduction in the CNS is facilitated by insulation of axons with myelin, a specialized oligodendroglial compartment distant from the cell body. Myelin is turned over and adapted throughout life; however, the molecular and cellular basis of myelin dynamics remains elusive. Here we performed a comprehensive transcriptome analysis (RNA-seq) of myelin biochemically purified from mouse brains at various ages and find a surprisingly large pool of transcripts enriched in myelin. Further computational analysis showed that the myelin transcriptome is closely related to the myelin proteome but clearly distinct from the transcriptomes of oligodendrocytes and brain tissues, suggesting a highly selective incorporation of mRNAs into the myelin compartment. The mRNA-pool in myelin displays maturation-dependent dynamic changes of composition, abundance, and functional associations; however ageing-dependent changes after 6 months were minor. We suggest that this transcript pool enables myelin turnover and the local adaptation of individual pre-existing myelin sheaths.

引用

页数：12

共 58 条

[1] Myelin Membrane Assembly Is Driven by a Phase Transition of Myelin Basic Proteins Into a Cohesive Protein Meshwork [J].