Identification and discrimination of Burkholderia pseudomallei, B-mallei, and B-thailandensis by real-time PCR targeting type III secretion system genes

被引:81
作者
Thibault, FM [1 ]
Valade, E [1 ]
Vidal, DR [1 ]
机构
[1] Ctr Rech, Serv Sante Armees, F-38702 La Tronche, France
关键词
D O I
10.1128/JCM.42.12.5871-5874.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.
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页码:5871 / 5874
页数:4
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