Identification and discrimination of Burkholderia pseudomallei, B-mallei, and B-thailandensis by real-time PCR targeting type III secretion system genes

被引：81

作者：

Thibault, FM ^{[1
]}

Valade, E ^{[1
]}

Vidal, DR ^{[1
]}

机构：

[1] Ctr Rech, Serv Sante Armees, F-38702 La Tronche, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 12期

关键词：

D O I：

10.1128/JCM.42.12.5871-5874.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

引用

页码：5871 / 5874

页数：4

共 22 条

[1] Bacterial flagella and type III secretion systems
Aizawa, S
[J]. FEMS MICROBIOLOGY LETTERS, 2001, 202 (02) : 157 - 164
[2] [Anonymous], 1995, CURRENT PROTOCOLS MO
[3] IDENTIFICATION OF PSEUDOMONAS-PSEUDOMALLEI IN THE CLINICAL LABORATORY
ASHDOWN, LR
[J]. JOURNAL OF CLINICAL PATHOLOGY, 1979, 32 (05) : 500 - 504
[4] Secretion of Ipa proteins by Shigella flexneri: Inducer molecules and kinetics of activation
Bahrani, FK
Sansonetti, PJ
Parsot, C
[J]. INFECTION AND IMMUNITY, 1997, 65 (10) : 4005 - 4010
[5] Type III secretion systems and bacterial flagella: Insights into their function from structural similarities
Blocker, A
Komoriya, K
Aizawa, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (06) : 3027 - 3030
[6] Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species
Brett, PJ
DeShazer, D
Woods, DE
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 : 317 - 320
[7] The invasion-associated type-III protein secretion system in Salmonella - A review
Collazo, CM
Galan, JE
[J]. GENE, 1997, 192 (01) : 51 - 59
[8] Evaluation of PCR for diagnosis of melioidosis
Haase, A
Brennan, M
Barrett, S
Wood, Y
Huffam, S
O'Brien, D
Currie, B
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) : 1039 - 1041
[9] Type III protein secretion systems in bacterial pathogens of animals and plants
Hueck, CJ
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (02) : 379 - +
[10] Automated identification systems and Burkholderia pseudomallei
Koh, TH
Ng, LSY
Ho, JLF
Sng, LH
Wang, GCY
Lin, RVTP
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) : 1809 - 1809

← 1 2 3 →