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A Microfluidic Microbeads Fluorescence Assay with Quantum Dots-Bead-DNA Probe
被引:4
作者:
Ankireddy, S. R.
[1
]
Kim, Jongsung
[1
]
机构:
[1] Gachon Univ, Dept Chem & Biol Engn, Songnam 461701, South Korea
基金:
新加坡国家研究基金会;
关键词:
Quantum Dots-Bead-DNA;
Microfluidic Chip;
N-Ras Gene;
TOTAL ANALYSIS SYSTEMS;
CHIP;
EXPRESSION;
PROTEINS;
GENES;
D O I:
10.1166/jnn.2016.11072
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
A microfluidic bead-based nucleic acid sensor for the detection of tumor causing N-Ras genes using quantum dots has been developed. Presently, quantum dots-bead-DNA probe based hybridization detection methods are often called as 'bead based assays' and their success is substantially influenced by the dispensing and manipulation capability of the microfluidic technology. This study reports the detection of N-Ras cancer gene by fluorescence quenching of quantum dots immobilized on the surface of polystyrene beads. A microfluidic chip was constructed in which the quantum dots-bead-DNA probes were packed in the channel. The target DNA flowed across the beads and hybridized with immobilized probe sequences. The target DNA can be detected by the fluorescence quenching of the quantum dots due to their transfer of emission energy to intercalation dye after DNA hybridization. The mutated gene also induces fluorescence quenching but with less degree than the perfectly complementary target DNA.
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页码:2897 / 2899
页数:3
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