Impact of Genome Reduction on Bacterial Metabolism and Its Regulation

被引：232

作者：

Yus, Eva ^{[2
,3
]}

Maier, Tobias ^{[2
,3
]}

Michalodimitrakis, Konstantinos ^{[2
,3
]}

van Noort, Vera ^{[1
]}

Yamada, Takuji ^{[1
]}

Chen, Wei-Hua ^{[1
]}

Wodke, Judith A. H. ^{[2
,3
]}

Gueell, Marc ^{[2
,3
]}

Martinez, Sira ^{[2
,3
]}

Bourgeois, Ronan ^{[2
,3
]}

Kuehner, Sebastian ^{[1
]}

Raineri, Emanuele ^{[2
,3
]}

Letunic, Ivica ^{[1
]}

Kalinina, Olga V. ^{[1
,4
]}

Rode, Michaela ^{[1
]}

Herrmann, Richard ^{[4
]}

Gutierrez-Gallego, Ricardo ^{[5
]}

Russell, Robert B. ^{[1
]}

Gavin, Anne-Claude ^{[1
]}

Bork, Peer ^{[1
]}

Serrano, Luis ^{[2
,3
,6
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] CRG, Barcelona 08003, Spain

[3] Univ Pompeu Fabra, Barcelona 08003, Spain

[4] Russian Acad Sci, Inst Informat Transmiss Problems, Moscow 127994, Russia

[5] ZMBH, D-69120 Heidelberg, Germany

[6] Inst Catalana Recerca & Estudis Avancats, Barcelona 08010, Spain

来源：

SCIENCE | 2009年 / 326卷 / 5957期

关键词：

MYCOPLASMA-PNEUMONIAE; LACTOCOCCUS-LACTIS; ESCHERICHIA-COLI; MOLLICUTES; GENES; ANNOTATION; SEQUENCE; GROWTH;

D O I：

10.1126/science.1177263

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and C-13-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.

引用

页码：1263 / 1268

页数：6

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