Differentiation of human umbilical cord-derived mesenchymal stem cells into hepatocytes in vitro

被引：15

作者：

Zheng, Guan ^{[1
]}

Liu, Yang ^{[1
]}

Jing, Qian ^{[1
]}

Zhang, Lei ^{[1
]}

机构：

[1] Kunming Med Univ, Affiliated Calmette Hosp, Biomed Res Ctr, Peoples Hosp Kunming 1, Kunming, Yunnan, Peoples R China

来源：

BIO-MEDICAL MATERIALS AND ENGINEERING | 2015年 / 25卷

关键词：

Human umbilical cord-derived mesenchymal stem cells (HUC-MSCs); hepatocytic differentiation; hepatocytes; BONE-MARROW; TISSUE; BLOOD;

D O I：

10.3233/BME-141249

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

OBJECTIVE: The stem cell based therapy is a potential alternative to liver transplantation. The aim of the study is to investigate the hepatocytic differentiation ability of human umbilical cord derived mesenchymal stem cells (HUC-MSCs) in vitro. METHODS: The HUC-MSCs were isolated from Wharton's jelly of human umbilical cord. The cells were identified by assessing the stem cell markers. The HUC-MSCs were characterized by multipotency of differentiation. We modified the hepatogenic differentiation protocol and examine the function of differentiated cell by Periodic acid-Schiff (PAS) staining and Low-Density Lipoprotein (LDL) uptake. The protein expressions of Total protein (TP), Albumin (ALB), Globulin (GLB), Urea (BUN) and a-fetoprotein (AFP) were also detected. RESULTS: The cells of the hepatogenic differentiation group showed the function of hepatocyte. Protein expressions of TP, ALB, GLB BUN and AFP improve that the HUC-MSCs are able to different into the functional hepatogenic-like cell. CONCLUSION: The above founding indicated that the HUC-MSCs are able to different into the functional hepatogenic-like cell by present method.

引用

页码：S145 / S157

页数：13

共 20 条

[1]

Alison MR, 2000, NATURE, V20, P406

[2] Stem cells in tissue engineering [J].

Bianco, P ;

Robey, PG .

NATURE, 2001, 414 (6859) :118-121

[3] Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood [J].

Bieback, K ;

Kern, S ;

Klüter, H ;

Eichler, H .

STEM CELLS, 2004, 22 (04) :625-634

[4]

[陈军 Chen Jun], 2010, [中国组织工程研究与临床康复, Journal of Clinical Rehabilitative Tissue Engineering Research], V14, P9257

[5] Dental pulp stem cells: A promising tool for bone regeneration [J].

d'Aquino, Riccardo ;

Papaccio, Gianpaolo ;

Laino, Gregorio ;

Graziano, Antonio .

STEM CELL REVIEWS, 2008, 4 (01) :21-26

[6] In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells'into hepatocyte-like cells [J].

Hong, SH ;

Gang, EJ ;

Jeong, JA ;

Ahn, CY ;

Hwang, SH ;

Yang, IH ;

Park, HK ;

Han, H ;

Kim, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 330 (04) :1153-1161

[7] Analysis of the mechanism for chromatin remodeling in embryos reconstructed by somatic nuclear transfer [J].

Kim, JM ;

Ogura, A ;

Nagata, M ;

Aoki, F .

BIOLOGY OF REPRODUCTION, 2002, 67 (03) :760-766

[8] Establishment, characterization, and long-term maintenance of cultures of human fetal hepatocytes [J].

Lázaro, CA ;

Croager, EJ ;

Mitchell, C ;

Campbell, JS ;

Yu, CP ;

Foraker, J ;

Rhim, JA ;

Yeoh, GCT ;

Fausto, N .

HEPATOLOGY, 2003, 38 (05) :1095-1106

[9]

Liu Lingying, 2011, Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, V25, P745

[10]

Liu Y, 2012, J ANIM VET ADV, V11, P1739

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