Quantitative Assessment of the Effects of Oxidants on Antigen-Antibody Binding In Vitro

被引：8

作者：

Han, Shuang ^{[1
]}

Wang, Guanyu ^{[2
]}

Xu, Naijin ^{[1
]}

Liu, Hui ^{[1
]}

机构：

[1] Dalian Med Univ, Coll Med Lab, Dalian 116044, Peoples R China

[2] Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China

来源：

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | 2016年 / 2016卷

关键词：

HUMORAL IMMUNE-RESPONSE; OXIDATIVE STRESS; INFLAMMATION; PROTEIN; CANCER;

D O I：

10.1155/2016/1480463

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective. We quantitatively assessed the influence of oxidants on antigen-antibody-binding activity. Methods. We used several immunological detection methods, including precipitation reactions, agglutination reactions, and enzyme immunoassays, to determine antibody activity. The oxidation-reduction potential was measured in order to determine total serum antioxidant capacity. Results. Certain concentrations of oxidants resulted in significant inhibition of antibody activity but had little influence on total serum antioxidant capacity. Conclusions. Oxidants had a significant influence on interactions between antigen and antibody, but minimal effect on the peptide of the antibody molecule.

引用

页数：7

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