Inhibition effect of procyanidins from lotus seedpod on mouse B16 melanoma in vivo and in vitro

被引：55

作者：

Duan, Yuqing ^{[1
]}

Zhang, Haihui ^{[1
]}

Xu, Feifei ^{[1
]}

Xie, Bijun ^{[2
]}

Yang, Xianwen ^{[3
]}

Wang, Yong ^{[1
]}

Yan, Yongsheng ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

[3] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China

来源：

FOOD CHEMISTRY | 2010年 / 122卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Lotus seedpod; Procyanidin; Melanoma; Anti-cancer activity; Apoptosis; Immunomodulatory activity; EXTRACT; APOPTOSIS; CELLS; PROANTHOCYANIDINS; PROLIFERATION; ASSAY;

D O I：

10.1016/j.foodchem.2010.02.020

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Significant growth inhibition effects of procyanidins from lotus (Nelumbo nucifera Gaertn.) seedpod (LSPCs) on mouse melanoma B16 were found both in vivo and in vitro. In vivo treatment with LSPCs inhibited tumour growth in C57BL/6 J mice by 55.3% in terms of average tumour weight. LSPCs can significantly (P < 0.05) decrease lipid peroxidation (LPO) levels and increase the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in liver tissue. In vitro assay of anti-cancer activities suggested that LSPCs (25-100 mu g/ml) possessed cytotoxicities against mouse melanoma B16 in a dose-dependent mode. Furthermore, LSPCs had significant (P<0.05) stimulatory effects on mouse splenocyte proliferation. The prevention of tumour growth was exerted through diverse mechanisms, including cell-cycle arrest, induction of tumour cell death by apoptosis and increase of Ca2+ ions, together with stimulation of antioxidant enzyme activities and immunomodulatory activities. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：84 / 91

页数：8

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