Characterization and Complete Genome Sequence of a Novel Siphoviridae Bacteriophage BS5

被引：10

作者：

Meng, Xue ^{[1
]}

Wang, Min ^{[1
,2
]}

You, Siyuan ^{[1
]}

Wang, Duobing ^{[1
]}

Li, Yan ^{[1
]}

Liu, Zhaoyang ^{[1
]}

Gao, Yu ^{[1
]}

Liu, Lu ^{[1
]}

Zhang, Yaoyuan ^{[1
]}

Yan, Zhenghao ^{[3
]}

Liu, Chunyan ^{[4
]}

Jiang, Yong ^{[1
,2
]}

Shao, Hongbing ^{[1
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

[2] Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China

[3] 1 Middle Sch, Pingdu 266700, Shandong, Peoples R China

[4] 58 Middle Sch, Qingdao 266003, Shandong, Peoples R China

来源：

CURRENT MICROBIOLOGY | 2017年 / 74卷 / 07期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Phage; Genomic; Siphoviridae family Phage; Yellow Sea; YELLOW SEA; PHAGE; VIRUSES; PROTEINS; SM9913; RANGE; LYSIS; MODEL; RNA; DNA;

D O I：

10.1007/s00284-017-1221-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel Siphoviridae family Phage BS5, which infects Pseudoalteromonas atlantica, was isolated from the surface waters of the Yellow Sea. Morphological study by transmission electron microscopy revealed that the novel phage belongs to Siphoviridae. The complete genome sequence of PBS5 contained a linear, double-strand 39949-bp DNA molecule with a G + C content of 40.6% and 65 putative open reading frames. Twelve conserved domains were detected by BLASTP in NCBI, and of these the functions of 5 were known. The genome was grouped into four modules as follows: phage structure, phage packaging, DNA replication and regulation, and some additional functions.16 S rDNA sequence analysis was also applied to identify the host bacteria. After initial characterization of bacteriophage PBS5, it was found that the optimal pH was 7.0, the optimal temperature was 30 A degrees C, and the burst size was about 95 virions per cell. This information will provide an important benchmark for further research on the interaction between bacteriophages and their hosts.

引用

页码：815 / 820

页数：6