Molecular and functional characterization of peptidoglycan-recognition protein SC2 (PGRP-SC2) from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae

被引：30

作者：

Gan, Zhen ^{[1
,2
,3
]}

Chen, Shannan ^{[1
,3
]}

Hou, Jing ^{[1
,3
]}

Huo, Huijun ^{[1
,3
]}

Zhang, Xiaolin ^{[1
,3
]}

Ruan, Baiye ^{[1
,3
]}

Laghari, Zubair Ahmed ^{[1
]}

Li, Li ^{[1
,3
]}

Lu, Yishan ^{[2
]}

Nie, Pin ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

[2] Guangdong Ocean Univ, Coll Fishery, 40 East Jiefang Rd, Zhanjiang 524025, Guangdong, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2016年 / 54卷

基金：

中国国家自然科学基金;

关键词：

Oreochromis niloticus; Peptidoglycan-recognition protein; Streptococcus agalactiae; Amidase activity; Antimicrobial effect; EXPRESSION; HISTORY; MENINGOENCEPHALITIS; AMIDASE; DEFENSE; PGRPS; FLY;

D O I：

10.1016/j.fsi.2016.03.158

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

PGRP-SC2, the member of PGRP family, plays an important role in regulation of innate immune response. In this paper, a PGRP-SC2 gene of Nile tilapia, Oreochromis niloticus (designated as On-PGRP-SC2) was cloned and its expression pattern under the infection of Streptococcus agalactiae was investigated. Sequence analysis showed main structural features required for amidase activity were detected in the deduced amino acid sequence of On-PGRP-SC2. In healthy tilapia, the On-PGRP-SC2 transcripts could be detected in all the examined tissues, with the most abundant expression in the muscle. When infected with S. agalactiae, there was a clear time-dependent expression pattern of On-PGRP-SC2 in the spleen, head kidney and brain. The assays for the amidase activity suggested that recombinant On-PGRP-SC2 protein had a Zn2+-dependent PGN-degrading activity. Moreover, our works showed that recombinant On-PGRP-SC2 protein could significantly reduce bacterial load in target organs attacked by S. agalactiae. These findings indicated that On-PGRP-SC2 may play important roles in the immune response to S. agalactiae in Nile tilapia. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1 / 10

页数：10

共 40 条

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