Molecular characterization and tissue expression profile of the Dnmts gene family in pig

被引：0

作者：

Luo Zong-gang ^{[1
,2
,3
]}

Zhang Kai ^{[4
]}

Chen Lei ^{[1
,3
]}

Yang Yuan-xin ^{[2
]}

Fu Peng-hui ^{[2
]}

Wang Ke-tian ^{[1
]}

Wang Ling ^{[2
]}

Li Ming-zhou ^{[5
]}

Li Xue-wei ^{[5
]}

Zuo Fu-yuan ^{[2
]}

Wang Jin-yong ^{[1
,3
]}

机构：

[1] Chongqing Acad Anim Sci, Chongqing 402460, Peoples R China

[2] Southwest Univ, Dept Anim Sci, Rongchang Campus, Chongqing 402460, Peoples R China

[3] Minist Agr, Key Lab Pig Ind Sci, Chongqing 402460, Peoples R China

[4] Sichuan Acad Grassland Sci, Chengdu 610000, Peoples R China

[5] Sichuan Agr Univ, Coll Anim Sci & Technol, Inst Anim Genet & Breeding, Chengdu 611130, Peoples R China

来源：

JOURNAL OF INTEGRATIVE AGRICULTURE | 2017年 / 16卷 / 06期

关键词：

pig; Dnmt gene family; cloning; mRNA expression; DNA METHYLTRANSFERASE; DE-NOVO; METHYLATION; MAMMALS; EMBRYOS; SEQUENCE; HOMOLOG; CLONING; CELLS; PCR;

D O I：

10.1016/S2095-3119(16)61512-5

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

DNA methyltransferases (Dnmts) comprise a family of proteins which involved in the establishment and maintenance of DNA methylation patterns. In pig, the molecular characterization and tissue expression profile of Dnmt gene family are not clear. To solve this problem, reverse transcriptase PCR and rapid amplification of cDNA ends were used to clone the sequences of the porcine Dnmt2 and Dnmt3b genes. Furthermore, the mRNA expression profiles of Dnmt1, Dnmt2, Dnmt3a and Dnmt3b genes from 54 adult tissues and 2 entire fetuses of Rongchang pig were analyzed by quantitative real-time PCR (qRT-PCR). As a result, the lengths of porcine Dnmt2 and Dnmt3b gene cDNAs were 1227 and 2559 bp with cytosine-C5 specific DNA methylase domain, respectively. The four Dnmt genes were highly expressed in longissimus dorsi muscle (P<0.01). Dnmtl is highly expressed in heart (P<0.01) and Dnmt2 shows its preference in liver and seminal vesicle tissue (P<0.01). Dnmt3a and Dnmt3b are highly expressed in the two fetus stages (P<0.01). All these results suggested that each gene has its specific expression profile, and deeper study is required to dig more details between the methylation level and Dnmt family mRNA expressions in different tissues.

引用

页码：1367 / 1374

页数：8

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