Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System

被引：29

作者：

An, S. S. A. ^{[1
]}

Lim, K. T. ^{[2
]}

Oh, H. J. ^{[2
]}

Lee, B. S. ^{[2
]}

Zukic, E. ^{[3
]}

Ju, Y. R. ^{[4
]}

Yokoyama, T. ^{[5
]}

Kim, S. Y. ^{[6
]}

Welker, E. ^{[3
,7
,8
]}

机构：

[1] Kyungwon Univ, Dept Bionano Technol, Songnam 461701, Kyungki Do, South Korea

[2] PeopleBio Inc, Dept Res & Dev, Seoul, South Korea

[3] Hungarian Acad Sci, Biol Res Ctr, Inst Biochem, H-6701 Szeged, Hungary

[4] KNIH CDC, Dept Neurovirol, Seoul, South Korea

[5] Prion Dis Res Ctr, Natl Inst Anim Hlth, Tsukuba, Ibaraki, Japan

[6] Seoul Natl Univ, Bundang Hosp, Ctr Neurosci, Coll Med, Seoul 151, South Korea

[7] Hungarian Acad Sci, Biol Res Ctr, Inst Enzymol, Budapest, Hungary

[8] Univ Szeged, Fac Sci & Informat, Dept Biochem & Mol Biol, Szeged, Hungary

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 392卷 / 04期

关键词：

TSE; Prion; Blood plasma; Detection; ELISA; Aggregation; Multimers; Monomer; Antibody; CREUTZFELDT-JAKOB-DISEASE; RECOMBINANT PRP; IMMUNOCAPILLARY ELECTROPHORESIS; SEEDED CONVERSION; ANTIBODIES; ASSAY; SHEEP;

D O I：

10.1016/j.bbrc.2010.01.053

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：505 / 509

页数：5

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