Consensus guidelines for the use and interpretation of angiogenesis assays

被引：501

作者：

Nowak-Sliwinska, Patrycja ^{[1
,2
]}

Alitalo, Kari ^{[3
]}

Allen, Elizabeth ^{[4
]}

Anisimov, Andrey ^{[3
]}

Aplin, Alfred C. ^{[5
]}

Auerbach, Robert ^{[6
]}

Augustin, Hellmut G. ^{[7
,8
,9
]}

Bates, David O. ^{[10
]}

van Beijnum, Judy R. ^{[11
]}

Bender, R. Hugh F. ^{[12
]}

Bergers, Gabriele ^{[4
,13
]}

Bikfalvi, Andreas ^{[14
]}

Bischoff, Joyce ^{[15
,16
]}

Boeck, Barbara C. ^{[7
,8
,9
]}

Brooks, Peter C. ^{[17
]}

Bussolino, Federico ^{[18
,19
]}

Cakir, Bertan ^{[20
]}

Carmeliet, Peter ^{[21
,22
,23
]}

Castranova, Daniel ^{[24
]}

Cimpean, Anca M. ^{[25
]}

Cleaver, Ondine ^{[26
]}

Coukos, George ^{[27
]}

Davis, George E. ^{[28
,29
]}

De Palma, Michele ^{[30
]}

Dimberg, Anna ^{[31
]}

Dings, Ruud P. M. ^{[32
]}

Djonov, Valentin ^{[33
]}

Dudley, Andrew C. ^{[34
,35
]}

Dufton, Neil P. ^{[36
]}

Fendt, Sarah-Maria ^{[37
,38
,39
]}

Ferrara, Napoleone ^{[40
]}

Fruttiger, Marcus ^{[41
]}

Fukumura, Dai ^{[42
,43
]}

Ghesquiere, Bart ^{[44
,45
]}

Gong, Yan ^{[20
]}

Griffin, Robert J. ^{[32
]}

Harris, Adrian L. ^{[46
]}

Hughes, Christopher C. W. ^{[12
]}

Hultgren, Nan W. ^{[12
]}

Iruela-Arispe, M. Luisa ^{[47
]}

Irving, Melita ^{[27
]}

Jain, Rakesh K. ^{[42
,43
]}

Kalluri, Raghu ^{[48
]}

Kalucka, Joanna ^{[21
,22
,23
]}

Kerbel, Robert S. ^{[49
]}

Kitajewski, Jan ^{[50
]}

Klaassen, Ingeborg ^{[51
,52
]}

Kleinmann, Hynda K. ^{[53
]}

Koolwijk, Pieter ^{[54
]}

Kuczynski, Elisabeth ^{[49
]}

机构：

[1] Univ Lausanne, Univ Geneva, Fac Sci, Mol Pharmacol Grp,Sch Pharmaceut Sci,CMU, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland

[2] Univ Geneva, Translat Res Ctr Oncohaematol, Geneva, Switzerland

[3] Univ Helsinki, Wihuri Res Inst & Translat Canc Biol Program, Helsinki, Finland

[4] Katholieke Univ Leuven, Dept Oncol, Lab Tumor Microenvironm & Therapeut Resistance, VIB Ctr Canc Biol, Louvain, Belgium

[5] Univ Washington, Dept Pathol, Seattle, WA 98195 USA

[6] Univ Wisconsin, Madison, WI USA

[7] Heidelberg Univ, Med Fac Mannheim, European Ctr Angiosci, Heidelberg, Germany

[8] German Canc Res Ctr, Div Vasc Oncol & Metastasis Res, Heidelberg, Germany

[9] German Canc Consortium, Heidelberg, Germany

[10] Univ Nottingham, Sch Med, Div Canc & Stem Cells, Nottingham, England

[11] Vrije Univ Amsterdam, Dept Med Oncol, Canc Ctr Amsterdam, Angiogenesis Lab,Med Ctr, Boelelaan 1117, NL-1081 HV Amsterdam, Netherlands

[12] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92717 USA

[13] Univ Calif San Francisco, Dept Neurol Surg, Brain Tumor Res Ctr,Dept Neurol Surg, Helen Diller Family Comprehens Canc Ctr, San Francisco, CA USA

[14] Univ Bordeaux, Angiogenesis & Tumor Microenvironm Lab, INSERM, U1029, Pessac, France

[15] Harvard Med Sch, Boston Childrens Hosp, Vasc Biol Program, Boston, MA USA

[16] Harvard Med Sch, Boston Childrens Hosp, Dept Surg, Boston, MA USA

[17] Maine Med Ctr Res Inst, Ctr Mol Med, Scarborough, ME USA

[18] Univ Torino, Dept Oncol, Turin, Italy

[19] Candiolo Canc Inst FPO IRCCS, I-10060 Candiolo, Italy

[20] Harvard Med Sch, Boston Childrens Hosp, Dept Ophthalmol, Boston, MA USA

[21] Katholieke Univ Leuven, Dept Oncol, Lab Angiogenesis & Vasc Metab, Leuven, Belgium

[22] Katholieke Univ Leuven, Leuven Canc Inst LKI, Leuven, Belgium

[23] VIB, Ctr Canc Biol, Lab Angiogenesis & Vasc Metab, Leuven, Belgium

[24] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Div Dev Biol, NIH, Bethesda, MD USA

[25] Victor Babes Univ Med & Pharm, Dept Microscop Morphol Histol, Angiogenesis Res Ctr, Timisoara, Romania

[26] Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Ctr Regenerat Sci & Med, Dallas, TX 75390 USA

[27] Univ Lausanne, Ludwig Inst Canc Res, Dept Oncol, Lausanne, Switzerland

[28] Univ Missouri, Sch Med, Dept Med Pharmacol & Physiol, Columbia, MO USA

[29] Dalton Cardiovasc Ctr, Columbia, MO USA

[30] Swiss Fed Inst Technol, Sch Life Sci, Lausanne, Switzerland

[31] Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden

[32] Univ Arkansas Med Sci, Dept Radiat Oncol, Little Rock, AR 72205 USA

[33] Univ Bern, Inst Anat, Bern, Switzerland

[34] Univ Virginia, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA USA

[35] Univ Virginia, Emily Cour Canc Ctr, Charlottesville, VA USA

[36] Imperial Coll London, Vasc Sci, Imperial Ctr Translat & Expt Med, Natl Heart & Lung Inst, London, England

[37] VIB Ctr Canc Biol, Lab Cellular Metab & Metab Regulat, Leuven, Belgium

[38] Katholieke Univ Leuven, Lab Cellular Metab & Metab Regulat, Dept Oncol, Leuven, Belgium

[39] Leuven Canc Inst, Leuven, Belgium

[40] Univ Calif San Diego, La Jolla, CA 92093 USA

[41] UCL, Inst Ophthalmol, London, England

[42] Massachusetts Gen Hosp, Dept Radiat Oncol, Edwin L Steele Labs, Boston, MA 02114 USA

[43] Harvard Med Sch, Boston, MA 02114 USA

[44] VIB, VIB Ctr Canc Biol, Metabol Expertise Ctr, Leuven, Belgium

[45] Katholieke Univ Leuven, Dept Oncol, Metabol Expertise Ctr, Leuven, Belgium

[46] Univ Oxford, John Radcliffe Hosp, Mol Oncol Labs, Weatherall Inst Mol Med,Dept Oncol, Oxford, England

[47] Univ Calif Los Angeles, MCDB, Los Angeles, CA USA

[48] Univ Texas MD Anderson Canc Ctr, Dept Canc Biol, Metastasis Res Ctr, Houston, TX 77030 USA

[49] Univ Toronto, Dept Med Biophys, Sunnybrook Res Inst, Biol Sci Platform, Toronto, ON, Canada

[50] Univ Illinois, Dept Physiol & Biophys, Chicago, IL 60680 USA

来源：

ANGIOGENESIS | 2018年 / 21卷 / 03期

基金：

美国国家卫生研究院; 欧洲研究理事会; 瑞士国家科学基金会;

关键词：

Angiogenesis; Aortic ring; Endothelial cell migration; Proliferation; Microfluidic; Zebrafish; Chorioallantoic membrane (CAM); Vascular network; Intussusceptive angiogenesis; Retinal vasculature; Corneal angiogenesis; Hindlimb ischemia; Myocardial angiogenesis; Recombinant proteins; Tip cells; Plug assay; Vessel co-option; ENDOTHELIAL-GROWTH-FACTOR; EMBRYO CHORIOALLANTOIC MEMBRANE; VASCULAR-PERMEABILITY FACTOR; PERIPHERAL ARTERIAL-DISEASE; AORTIC RING MODEL; VESSEL CO-OPTION; PANCREATIC NEUROENDOCRINE TUMORS; OXYGEN-INDUCED RETINOPATHY; LIVING CAPILLARY NETWORKS; ETS TRANSCRIPTION FACTORS;

D O I：

10.1007/s10456-018-9613-x

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.

引用

页码：425 / 532

页数：108

共 713 条

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