Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

被引：6

作者：

Kamei, K ^{[1
]}

Haruyama, T ^{[1
]}

Mie, M ^{[1
]}

Yanagida, Y ^{[1
]}

Kobatake, E ^{[1
]}

Aizawa, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 04期

基金：

日本学术振兴会;

关键词：

cellular biosensing system; inducible nitric oxide synthase; lipopolysaccharide; macrophage cell line; nitric oxide; polyion complex;

D O I：

10.1023/A:1022339929026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.

引用

收藏

页码：321 / 325

页数：5

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