Simultaneous extraction and separation of oil, proteins, and glucosinolates from Moringa oleifera seeds

被引：48

作者：

Chen, Rui ^{[1
]}

Wang, Xiu-Juan ^{[1
]}

Zhang, Yao-Yuan ^{[1
]}

Xing, Yan ^{[1
]}

Yang, Liu ^{[1
]}

Ni, He ^{[1
]}

Li, Hai-Hang ^{[1
,2
]}

机构：

[1] South China Normal Univ, Sch Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Guangdong, Peoples R China

[2] Huizhou Univ, Sch Life Sci, Huizhou 516007, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 300卷

关键词：

Moringa oleifera seeds; Oil; Proteins; Glucosinolates; Extraction; Separation; COLUMN CHROMATOGRAPHIC EXTRACTION; COAGULANT PROTEIN; ISOTHIOCYANATE; PURIFICATION; SOLVENT; TREE;

D O I：

10.1016/j.foodchem.2019.125162

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Moringa oleifera is a worldwide cultivated edible and medicinal plant. Its seeds are rich in oil, proteins, and glucosinolates. A practical method was developed to simultaneously extract and separate the three groups of substances from M. oleifera seeds. Smashed seed material was loaded into columns with petroleum ether: ethanol 8:2 (PE-ethanol) and eluted sequentially with 4.8-fold PE-ethanol to extract oil, and 10.8-fold water to extract proteins and glucosinolates. More than 95% of oil, proteins, and glucosinolates were extracted. The extracts were separated automatically into ether (oil) phase and ethanol aqueous phase. The latter was further separated into proteins and glucosinolates by 70% ethanol precipitation. The main glucosinolate was identified by LC-MS as GLC (4-alpha-rhamnopyranosyloxy-benzyl glucosinolate). After purification, 22.3 g refined oil, 33.0 g proteins, and 5.5 g purified GLC from 100 g M. oleifera seeds were obtained. This study provides a simple and high-efficient method to utilize M. oleifera seeds.

引用

页数：8

共 39 条

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