Anti-virus activity induced by polyinosinic:polycytidylic acid copolymer in in vitro chick embryo fibroblast cells

被引：1

作者：

Lv Yingjun ^{[1
]}

Bao Endong ^{[1
]}

Hartung, Joerg ^{[2
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

[2] Univ Vet Med Hannover, Hannover, Germany

来源：

BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT | 2010年 / 123卷 / 5-6期

关键词：

polyinosinic:polycytidylic acid copolymer; Newcastle disease virus; anti-virus activity; IFN; toxicity; DOUBLE-STRANDED-RNA; TOLL-LIKE RECEPTOR-3; NF-KAPPA-B; POLYRIBOCYTIDYLIC ACID; INTERFERON INDUCTION; APOPTOSIS; ACTIVATION; MECHANISMS; INFECTION; NECROSIS;

D O I：

10.2376/0005-9366-123-210

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The effect of a polyinosinic:polycytidylic acid copolymer (Poly I:C) on chick embryo fibroblast (CEF) cell viability, anti-Newcastle disease virus (NDV) activity and on IFN-alpha and IFN-beta levels were determined in vitro. Poly I:C inhibited NDV growth in CEF cells in the range of 30-500 mu g/mL and over 0.75-12 h of treatment. The anti-NDV activity was maximal for 60 mu g/mL of Poly I:C treatment at 12 h, after which it declined. The result of VSV protection assay which was used to determine IFN-activity showed that Poly I:C inhibited VSV growing on CEF cells, indicating that Poly I:C had immune-stimulating activities. The amount of IFN-beta in cell supernatant and the transcription levels of IFN-alpha mRNA and IEN-beta mRNA increased after Poly I:C treatment, indicating that the anti-viral activity related to the production of type I IFN. However Poly I:C also showed a toxic effect by inhibiting CEF cell proliferation in a dose-dependent and time-dependent manner. The results of this study indicate that the dosage level of Poly I:C must be carefully considered when used in a clinical setting.

引用

页码：210 / 214

页数：5

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