Oligonucleotide ligation assay for detecting mutations in the human immunodeficiency virus type 1 pol gene that are associated with resistance to zidovudine, didanosine, and lamivudine

被引：64

作者：

Edelstein, RE

Nickerson, DA

Tobe, VO

Manns-Arcuino, LA

Frenkel, LM

机构：

[1] Univ Washington, Dept Pediat, Div Infect Dis, Seattle, WA 98195 USA

[2] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 02期

关键词：

D O I：

10.1128/JCM.36.2.569-572.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This report describes the detection of mutations in the pol gene of human immunodeficiency virus type 1 associated with resistance to zidovudine, didanosine, and lamivudine by genotyping by an oligonucleotide ligation assay specific codons in the pol gene amplified by PCR. Our studies demonstrate the sensitivity, simplicity, and specificity of this genotyping system.

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收藏

页码：569 / 572

页数：4

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