In Vivo Dynamic Deformation of Articular Cartilage in Intact Joints Loaded by Controlled Muscular Contractions

被引:17
作者
Abusara, Ziad [1 ]
Von Kossel, Markus [1 ]
Herzog, Walter [1 ]
机构
[1] Univ Calgary, Human Performance Lab, Fac Kinesiol, Calgary, AB T2N 1N4, Canada
基金
加拿大健康研究院;
关键词
MECHANICAL-PROPERTIES; CHONDROCYTE; COMPRESSION; EXERCISE; CELL; INDENTATION; BEHAVIOR;
D O I
10.1371/journal.pone.0147547
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
When synovial joints are loaded, the articular cartilage and the cells residing in it deform. Cartilage deformation has been related to structural tissue damage, and cell deformation has been associated with cell signalling and corresponding anabolic and catabolic responses. Despite the acknowledged importance of cartilage and cell deformation, there are no dynamic data on these measures from joints of live animals using muscular load application. Research in this area has typically been done using confined and unconfined loading configurations and indentation testing. These loading conditions can be well controlled and allow for accurate measurements of cartilage and cell deformations, but they have little to do with the contact mechanics occurring in a joint where non-congruent cartilage surfaces with different material and functional properties are pressed against each other by muscular forces. The aim of this study was to measure in vivo, real time articular cartilage deformations for precisely controlled static and dynamic muscular loading conditions in the knees of mice. Fifty and 80% of the maximal knee extensor muscular force (equivalent to approximately 0.4N and 0.6N) produced average peak articular cartilage strains of 10.5+/-1.0% and 18.3+/-1.3% (Mean+/-SD), respectively, during 8s contractions. A sequence of 15 repeat, isometric muscular contractions (0.5s on, 3.5s off) of 50% and 80% of maximal muscular force produced cartilage strains of 3.0+/-1.1% and 9.6+/-1.5% (Mean +/- SD) on the femoral condyles of the mouse knee. Cartilage thickness recovery following mechanical compression was highly viscoelastic and took almost 50s following force removal in the static tests.
引用
收藏
页数:12
相关论文
共 39 条
[1]   Muscular loading of joints triggers cellular secretion of PRG4 into the joint fluid [J].
Abusara, Z. ;
Krawetz, R. ;
Steele, B. ;
DuVall, M. ;
Schmidt, T. ;
Herzog, W. .
JOURNAL OF BIOMECHANICS, 2013, 46 (07) :1225-1230
[2]   A novel method for determining articular cartilage chondrocyte mechanics in vivo [J].
Abusara, Z. ;
Seerattan, R. ;
Leumann, A. ;
Thompson, R. ;
Herzog, W. .
JOURNAL OF BIOMECHANICS, 2011, 44 (05) :930-934
[3]   INTERSPECIES COMPARISONS OF INSITU INTRINSIC MECHANICAL-PROPERTIES OF DISTAL FEMORAL CARTILAGE [J].
ATHANASIOU, KA ;
ROSENWASSER, MP ;
BUCKWALTER, JA ;
MALININ, TI ;
MOW, VC .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1991, 9 (03) :330-340
[4]  
Buschmann MD, 1996, J CELL SCI, V109, P499
[5]   Zonal changes in the three-dimensional morphology of the chondron under compression: The relationship among cellular, pericellular, and extracellular deformation in articular cartilage [J].
Choi, Jae Bong ;
Youn, Inchan ;
Cao, Li ;
Leddy, Holly A. ;
Gilchrist, Christopher L. ;
Setton, Lori A. ;
Guilak, Farshid .
JOURNAL OF BIOMECHANICS, 2007, 40 (12) :2596-2603
[6]   In vivo measures of cartilage deformation: patterns in healthy and osteoarthritic female knees using 3T MR imaging [J].
Cotofana, Sebastian ;
Eckstein, Felix ;
Wirth, Wolfgang ;
Souza, Richard B. ;
Li, Xiaojuan ;
Wyman, Bradley ;
Hellio-Le Graverand, Marie-Pierre ;
Link, Thomas ;
Majumdar, Sharmila .
EUROPEAN RADIOLOGY, 2011, 21 (06) :1127-1135
[7]   Functional analysis of articular cartilage deformation, recovery, and fluid flow following dynamic exercise in vivo [J].
Eckstein, F ;
Tieschky, M ;
Faber, S ;
Englmeier, KH ;
Reiser, M .
ANATOMY AND EMBRYOLOGY, 1999, 200 (04) :419-424
[8]   The effects of exercise on human articular cartilage [J].
Eckstein, F ;
Hudelmaier, M ;
Putz, R .
JOURNAL OF ANATOMY, 2006, 208 (04) :491-512
[9]   In vivo cartilage deformation after different types of activity and its dependence on physical training status [J].
Eckstein, F ;
Lemberger, B ;
Gratzke, C ;
Hudelmaier, M ;
Glaser, C ;
Englmeier, KH ;
Reiser, M .
ANNALS OF THE RHEUMATIC DISEASES, 2005, 64 (02) :291-295
[10]   Patellar cartilage deformation in vivo after static versus dynamic loading [J].
Eckstein, F ;
Lemberger, B ;
Stammberger, T ;
Englmeier, KH ;
Reiser, M .
JOURNAL OF BIOMECHANICS, 2000, 33 (07) :819-825