Oxygen and mammalian cell culture: are we repeating the experiment of Dr. Ox?

被引：84

作者：

Ast, Tslil ^{[1
,2
,3
,4
]}

Mootha, Vamsi K. ^{[1
,2
,3
,4
]}

机构：

[1] Broad Inst, Cambridge, MA 02142 USA

[2] Massachusetts Gen Hosp, Howard Hughes Med Inst, Boston, MA 02114 USA

[3] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[4] Harvard Med Sch, Boston, MA 02115 USA

来源：

NATURE METABOLISM | 2019年 / 1卷 / 09期

关键词：

LIFE-SPAN; HYPOXIA; PHYSIOLOGY; DOMAIN;

D O I：

10.1038/s42255-019-0105-0

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Mammalian cell culture represents a cornerstone of modern biomedical research. There is growing appreciation that the media conditions in which cells are cultured can profoundly influence the observed biology and reproducibility. Here, we consider a key but often ignored variable, oxygen, and review why being mindful of this environmental parameter is so important in the design and interpretation of cell culture studies.

引用

收藏

页码：858 / 860

页数：3

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