Diagnostic system for rapid and sensitive differential detection of pathogens

被引：118

作者：

Briese, T

Palacios, G

Kokoris, M

Jabado, O

Liu, ZQ

Renwick, N

Kapoor, V

Casas, I

Pozo, F

Limberger, R

Perez-Brena, P

Ju, JY

Lipkin, WI

机构：

[1] Columbia Univ, Mailman Sch Publ Hlth, New York, NY 10032 USA

[2] Qiagen Inc, Valencia, CA USA

[3] Inst Salud Carlos III, Madrid, Spain

[4] New York State Dept Hlth, Albany, NY USA

来源：

EMERGING INFECTIOUS DISEASES | 2005年 / 11卷 / 02期

关键词：

D O I：

10.3201/eid1102.040492

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Naturally emerging and deliberately released pathogens demand new detection strategies to allow early recognition and containment. We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples.

引用

收藏

页码：310 / 313

页数：4

相关论文

共 12 条

[1] DETECTION OF ELECTROPHORE-LABELED DNA AND ALBUMIN BY GAS-CHROMATOGRAPHY - LABILE AMIDE ELECTROPHORIC RELEASE TAGS [J].

ABDELBAKY, S ;

ALLAM, K ;

GIESE, RW .

ANALYTICAL CHEMISTRY, 1993, 65 (04) :498-499

[2] Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays [J].

Coiras, MT ;

Aguilar, JC ;

García, ML ;

Casas, I ;

Pérez-Breña, P .

JOURNAL OF MEDICAL VIROLOGY, 2004, 72 (03) :484-495

[3] Molecular diagnosis of influenza [J].

Ellis, JS ;

Zambon, MC .

REVIEWS IN MEDICAL VIROLOGY, 2002, 12 (06) :375-389

[4] Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction enzyme hybridization assay (Hexaplex) [J].

Fan, J ;

Henrickson, KJ ;

Savatski, LL .

CLINICAL INFECTIOUS DISEASES, 1998, 26 (06) :1397-1402

[5]

Gröndahl B, 1999, J CLIN MICROBIOL, V37, P1

[6]

Kokoris M, 2000, MOL DIAGN, V5, P329

[7] OLIGODEOXYRIBONUCLEOTIDES WITH CONJUGATED DIHYDROPYRROLOINDOLE OLIGOPEPTIDES - PREPARATION AND HYBRIDIZATION PROPERTIES [J].

LUKHTANOV, EA ;

KUTYAVIN, IV ;

GAMPER, HB ;

MEYER, RB .

BIOCONJUGATE CHEMISTRY, 1995, 6 (04) :418-426

[8] 4-(TRIFLUOROMETHYL)-2,3,5,6-TETRAFLUOROBENZYL BROMIDE AS A NEW ELECTROPHORIC DERIVATIZING REAGENT [J].

SAHA, M ;

SAHA, J ;

GIESE, RW .

JOURNAL OF CHROMATOGRAPHY, 1993, 641 (02) :400-404

[9] Multiplex PCR for typing and subtyping influenza and respiratory syncytial viruses [J].

Stockton, J ;

Ellis, JS ;

Saville, M ;

Clewley, JP ;

Zambon, MC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (10) :2990-2995

[10] Improved utility of photolabile solid phase synthesis supports for the synthesis of oligonucleotides containing 3'-hydroxyl termini [J].

Venkatesan, H ;

Greenberg, MM .

JOURNAL OF ORGANIC CHEMISTRY, 1996, 61 (02) :525-529