A combined study of behavior and Fos expression in limbic structures after re-testing Wistar rats in the elevated plus-maze

被引:24
作者
Galvis-Alonso, O. Y. [1 ]
Garcia, A. M. B. [5 ]
Orejarena, M. J. [1 ,2 ]
Lamprea, M. R. [3 ]
Botelho, S. [4 ]
Conde, C. A. [2 ]
Morato, S.
Garcia-Cairasco, N. [1 ]
机构
[1] Univ Sao Paulo, Neurophysiol & Expt Neuroethol Lab, FMRP, BR-14049900 Sao Paulo, Brazil
[2] Univ Ind Santander, Escuela Med, Lab Neurofisiol, Bucaramanga, Colombia
[3] Univ Nacl Colombia, Fac Ciencias Humanas, Dept Psicol, Bogota, Colombia
[4] Univ Pontificia Bolivariana, Escuela Ciencias Sociales, Lab Nurociencias & Comportamiento, Bucaramanga, Colombia
[5] Univ Sabana, Fac Psicol, Lab Bases Biol Comportamiento, Bogota, Colombia
关键词
Anxiety; Hypothalamus; Septal nucleus; Memory; Fos protein; ONE-TRIAL TOLERANCE; ANIMAL-MODELS; ANXIETY; CHLORDIAZEPOXIDE; STIMULATION; INDUCTION; EXPOSURE;
D O I
10.1016/j.brainresbull.2010.01.007
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The elevated plus-maze is an animal model used to study anxiety. In a second session, rats show a reduction in the exploratory behavior even when the two sessions are separated by intervals as large as 7 days. The aim of the present study was to investigate whether the reduction in the exploratory behavior is maintained after intervals larger than 7 days. Additionally, we aimed at investigating eventual correlations between behaviors in the plus-maze and activation of limbic structures as measured by Fos protein expression after the second session. Rats were tested for 5 min in the elevated plus-maze and re-tested 3, 9 or 33 days later. Other groups were tested only once. The rat brains were processed for immunohistochemical detection of Fos protein. The results show a decrease in the open arms exploration in the second trial with intervals of 3, 9 and 33 days. The expression of Fos protein in the piriform cortex, septal nucleus and paraventricular hypothalamic nucleus in the groups tested with intervals of 9 and 33 days were statistically different from the other groups. The alterations observed in exploratory behavior in the second session in the plus-maze did not correlate with Fos expression. In conclusion, although the specific test conditions were sufficient to evoke behavioral alterations in exploration in the elevated plus-maze, they were enough to induce significant Fos protein expression in piriform cortex, septal nucleus and thalamic and hypothalamic paraventricular nuclei but not in other areas such as dorsomedial nucleus of the hypothalamus and amygdala nuclei, known to be also active participants in circuits controlling fear and anxiety. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:595 / 599
页数:5
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