Ribosomal external and internal transcribed spacers:: Combined use in the phylogenetic analysis of Medicago (Leguminosae)

被引：99

作者：

Bena, G

Jubier, MF

Olivieri, I

Lejeune, B

机构：

[1] Univ Montpellier 2, Inst Sci Evolut, F-34095 Montpellier 05, France

[2] Univ Paris 11, Inst Biotechnol Plantes, F-91405 Orsay, France

[3] INRA, Lab Ressources Genet & Ameliorat Luzernes Mediter, F-34130 Mauguio, France

来源：

JOURNAL OF MOLECULAR EVOLUTION | 1998年 / 46卷 / 03期

关键词：

phylogeny; ETS; ITS; Medicago;

D O I：

10.1007/PL00006306

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have estimated the potential phylogenetic utility of the ribosomal external transcribed spacer (ETS) from the nuclear ribosomal region. The ETS was sequenced from 13 annual Medicago (Fabaceae) species upstream a highly conserved motive which was found among many different organisms. In the genus Medicago, the ETS was found to evolve 1.5 times faster than the internal transcribed spacer and to be 1.5 times more informative. Reduced ribosomal maturation process constraints on ETS are proposed to explain the different evolutionary rates between the two spacers. Maximal phylogenetic resolution and support was obtained when the two spacers were analyzed together. No incongruence between the two spacers was found and ETS appears to be a valuable source of information for solidifying ITS plant phylogeny. The phylogeny obtained in Medicago suggests that none of the three subsections included in the study is monophyletic.

引用

页码：299 / 306

页数：8

共 43 条

[41] EVOLUTIONARILY CONSERVED STRUCTURAL ELEMENTS ARE CRITICAL FOR PROCESSING OF INTERNAL TRANSCRIBED SPACER-2 FROM SACCHAROMYCES-CEREVISIAE PRECURSOR RIBOSOMAL-RNA [J].